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However, achieving real\u2010time gene analysis while maintaining cell viability remains a persistent challenge in existing methodologies. In this work, a Nano\u2010Electro\u2010Sampling and Intelligent Analysis (Nesta) system is developed, which incorporates a portable biochip for living cell sampling and on\u2010chip gene identification with an intelligent image analysis program for accurate gene quantification. The sampling processing utilizes nanopore\u2010focused electric fields to safely perforate cell membrane and extract intracellular genes. Subsequent sample transfer is effectively realized by chip rotation, which minimizes sample loss and contamination. The detachable detection area satisfies both temperature requirements for gene amplification and cell viability maintenance for sequential sampling. Integrated with intelligent image analysis program, this system enables precise quantitative analysis of smartphone\u2010captured images. 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