{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T19:42:15Z","timestamp":1787427735660,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"49","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly lethal zoonotic virus with a maximum mortality rate of 30%. Despite its risks and potential for human\u2010to\u2010human transmission, the standard diagnostic method has been absent for SFTSV detection. Therefore, this study introduces a versatile SFTSV biosensing technology using the electrochemical\u2010clustered regularly interspaced short palindromic repeat (EC\u2010CRISPR) system. The sensing membrane is functionalized with the 2WJ DNA@Au\u2010MoS\n                    <jats:sub>2<\/jats:sub>\n                    signal probe as a strategy to amplify the EC response resulting from target detection events of the CRISPR system. The sensor exhibits selective, sensitive, and reproducible detection capabilities in phosphate\u2010buffered saline, human serum, and\n                    <jats:italic>Haemaphysalis longicornis<\/jats:italic>\n                    genomic DNA diluted conditions with detection limits of 210.7, 189.0, and 285.1 fM, respectively. This verifies the versatility of the fabricated system, which significantly contributes to the early SFTSV detection in various fields. In the meantime, the sufficient sensing performance is demonstrated in identifying of SFTSV from infectious agent DNA. Furthermore, the proposed EC\u2010CRISPR biosensing platform can be considered as a flexible foundational technique for the diagnosis of zoonotic infectious diseases, as it demonstrates practical applicability for the detection of\n                    <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n                    by utilizing a customized CRISPR system programming strategy.\n                  <\/jats:p>","DOI":"10.1002\/smll.202506860","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T09:29:16Z","timestamp":1761902956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Versatile Biosensing Tool: CRISPR\u2010Cas12a System\u2010Integrated Electrochemical Biosensor for Severe Fever with Thrombocytopenia Syndrome Virus Detection in Clinical and Environmental Conditions"],"prefix":"10.1002","volume":"21","author":[{"given":"Daehyeon","family":"Yoo","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8176-0460","authenticated-orcid":false,"given":"Hyunjun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayeon","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gahyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Vector Entomology Kyungpook National University  2559 Gyeongsang\u2010daero Sangju 37224 Republic of Korea"},{"name":"Research Institute of Invertebrate Vector Kyungpook National University  2559 Gyeongsang\u2010daero Sangju 37224 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoseph","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongyun","family":"An","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunggu","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulhwan","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Integrative Engineering Chung\u2010Ang University  Heukseok\u2010dong, Dongjak\u2010gu Seoul 06910 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4158-130X","authenticated-orcid":false,"given":"Taek","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Kwangwoon University  20 Kwangwoon\u2010Ro, Nowon\u2010Gu Seoul 01897 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26072"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06536"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1776"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-021-00610-1"},{"key":"e_1_2_8_5_1","first-page":"1","volume":"50","author":"Yang T.","year":"2022","journal-title":"Int. J. Mol. Med."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit100"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir804"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir776"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268820002290"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-022-00693-x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130706"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antib12010018"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007984"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104353"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202208198"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2021.114092"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13765-023-00771-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202214987"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112100"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0318742"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bsheal.2021.02.002"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13020314"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2025.107625"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2024.2356505"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-013-3901-4"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.012933"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0382-0"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3609"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202402402"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.131060"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1RA07962J"},{"key":"e_1_2_8_32_1","first-page":"91","volume":"31","author":"Newbury D. E.","year":"2009","journal-title":"J. Scann. Microsc."},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201502582"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apcatb.2020.118931"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2018.11.028"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1010501"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.02.025"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b01020"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115474"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201510219"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113019"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.09.038"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae613"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1351\/pac199769020297"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2023.134295"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac501786u"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08860-4"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01604-19"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-020-00257-5"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2009.05.009"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006062"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.4103\/apjtm.apjtm_95_24"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01298-08"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00742-14"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.09.031"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2020.101580"},{"key":"e_1_2_8_58_1","volume":"16","author":"Park B. J.","year":"2022","journal-title":"PLoS Negl. Trop. Dis."},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004595"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.3588"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.8b02366"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01931-12"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117607"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202417206"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4NR01618A"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c01373"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2023.133342"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.121878"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114671"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201905311"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116919"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4TB01624F"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114159"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2008.03.012"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202506860","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T11:36:38Z","timestamp":1765452998000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202506860"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,31]]},"references-count":74,"journal-issue":{"issue":"49","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/smll.202506860"],"URL":"https:\/\/doi.org\/10.1002\/smll.202506860","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,31]]},"assertion":[{"value":"2025-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e06860"}}