{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T11:54:56Z","timestamp":1765454096832,"version":"3.46.0"},"reference-count":43,"publisher":"Wiley","issue":"49","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JCYJ20220818103207016"],"award-info":[{"award-number":["JCYJ20220818103207016"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515010016"],"award-info":[{"award-number":["2025A1515010016"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2025,12]]},"abstract":"Abstract<\/jats:title>\n \n The uterine vascular system is essential for female reproductive function, but its structural complexity challenges conventional two\u2010dimensional (2D) analysis. Herein, a three\u2010dimensional (3D) fluorescence angiography strategy is developed using aggregation\u2010induced emission (AIE) based nanoparticles (NPs) combined with two\u2010photon microscopy. An AIE fluorophore (AIEgen\n AF<\/jats:bold>\n ) is developed, exhibiting near\u2010infrared emission at 715 nm.\n \n AF@SiO\n 2<\/jats:sub>\n NPs\n <\/jats:bold>\n \u00a0is engineered by encapsulating AIEgen\n AF<\/jats:bold>\n within a hydrophobic silica core. Compared to micellar counterpart (\n AF@F127 NPs<\/jats:bold>\n ),\n \n AF@SiO\n 2<\/jats:sub>\n NPs\n <\/jats:bold>\n exhibits enhanced fluorescence performance: a 2.99\u2010fold higher quantum yield (48.76%) and a 4.37\u2010fold greater two\u2010photon absorption cross\u2010section (266.56 GM). The silica surface of\n \n AF@SiO\n 2<\/jats:sub>\n NPs\n <\/jats:bold>\n is further coated with dextran to improve biocompatibility and \u201cstealth properties\u201d in blood circulation. The obtained\n \n AF@SiO\n 2<\/jats:sub>\n \u2010Dex NPs\n <\/jats:bold>\n show minimal cytotoxicity and systemic toxicity. For in vivo imaging, a minimally invasive device is developed to expose the murine uterus. Following intravenous administration of\n \n AF@SiO\n 2<\/jats:sub>\n \u2010Dex NPs\n <\/jats:bold>\n , the uterine vascular network and collagen fibers are visualized using two\u2010photon excitation fluorescence (TPEF) and second harmonic generation (SHG), respectively. This approach achieves an effective imaging depth exceeding 450 \u00b5m in tissue (signal\u2010to\u2010noise ratio >3). Key vascular parameters, such as vessel morphology, density, blood flow, and permeability, are systematacially analyzed.\n <\/jats:p>","DOI":"10.1002\/smll.202507998","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T10:16:27Z","timestamp":1762424187000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Two\u2010Photon 3D Fluorescence Angiography of Uterus by Silica Enhanced Aggregation\u2010Induced Emission Nanoparticles"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3504-775X","authenticated-orcid":false,"given":"Gang","family":"Feng","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Reproductive Immunology for Peri\u2010implantation Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics Shenzhen Zhongshan Obstetrics & Gynecology Hospital (formerly Shenzhen Zhongshan Urology Hospital) Shenzhen 518120 China"},{"name":"Guangdong Engineering Technology Research Center of Reproductive Immunology for Peri\u2010implantation Shenzhen 518120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8657-849X","authenticated-orcid":false,"given":"Miaozhuang","family":"Fan","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging School of Biomedical Engineering Shenzhen University Health Science Center Shenzhen 518055 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Reproductive Immunology for Peri\u2010implantation Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics Shenzhen Zhongshan Obstetrics & Gynecology Hospital (formerly Shenzhen Zhongshan Urology Hospital) Shenzhen 518120 China"},{"name":"Guangdong Engineering Technology Research Center of Reproductive Immunology for Peri\u2010implantation Shenzhen 518120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2506-3927","authenticated-orcid":false,"given":"Zhengzheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Industrial and Systems Engineering The Hong Kong Polytechnic University Hong Kong 999077 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4119-7337","authenticated-orcid":false,"given":"Mingyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging School of Biomedical Engineering Shenzhen University Health Science Center Shenzhen 518055 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanghua","family":"Wu","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Reproductive Immunology for Peri\u2010implantation Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics Shenzhen Zhongshan Obstetrics & Gynecology Hospital (formerly Shenzhen Zhongshan Urology Hospital) Shenzhen 518120 China"},{"name":"Guangdong Engineering Technology Research Center of Reproductive Immunology for Peri\u2010implantation Shenzhen 518120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilin","family":"Zou","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Reproductive Immunology for Peri\u2010implantation Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics Shenzhen Zhongshan Obstetrics & Gynecology Hospital (formerly Shenzhen Zhongshan Urology Hospital) Shenzhen 518120 China"},{"name":"Guangdong Engineering Technology Research Center of Reproductive Immunology for Peri\u2010implantation Shenzhen 518120 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