{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:51:39Z","timestamp":1772995899522,"version":"3.50.1"},"reference-count":67,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Muscle disorders such as myofibrillar myopathies and Duchenne muscular dystrophy involve mutations in key cytoskeletal proteins and lead to progressive muscle degeneration. Yet, the mechanical characterization of affected muscle cells has relied mainly on immature or non\u2010human models. Here, we introduce a human in vitro platform based on patient\u2010derived immortalized myoblasts differentiated into myotubes on nanogrooved substrates, which promote alignment and organotypic maturation. Using immunostaining and atomic force microscopy (AFM), we show that desmin\u2010 and dystrophin\u2010mutated myotubes exhibit distinct morphological and mechanical phenotypes compared to wild\u2010type myotubes. We developed an AFM stiffness pipeline to quantify cell body stiffness across myotubes of variable thickness. Desmin\u2010 and dystrophin\u2010mutated myotubes are stiffer than controls, with desmin mutants also displaying cytoskeletal disorganization. A dynamic fatigue assay (cyclic AFM indentations over time) further revealed impaired stiffening and faster mechanical fatigue in desmin mutants, while dystrophin mutants preserved resilience. This set of results establishes a reproducible and human\u2010relevant system to probe muscle mechanics in disease, offering a unique intermediate model between conventional immortalized lines and complex iPSC\u2010derived tissues, and enabling future quantitative screening and translational applications.<\/jats:p>","DOI":"10.1002\/smll.202511234","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T15:09:59Z","timestamp":1768230599000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genotype\u2010Dependent Morpho\u2010Mechanical Profiling of Patient\u2010Derived Human Myotubes on Nanogrooved Substrates"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4973-4246","authenticated-orcid":false,"given":"Rapha\u00ebl","family":"Cr\u00e9pin","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay Univ Evry CY Cergy Paris 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