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Dendritic cell (DC) vaccines have shown considerable potential for modulating injury\u2010induced immune dysregulation. However, their therapeutic efficacy is limited by factors such as restricted cellular viability, delayed onset of action, and lack of sustained immune activity. Therefore, to address these challenges, a hybrid hydrogel composed of a methacrylate\u2010modified decellularized lymph node extracellular matrix (DLMMA) and porous GelMA (pGelMA) carrying a neuroprotective DC (npDC) vaccine was developed in the form of cryomicroneedles (pG\/DL@npDC\u2010cryoMNs), enabling efficient and sustained immunological regulation during SCI treatment. The pG\/DL@npDC\u2010cryoMNs enhanced the viability of npDC vaccine and facilitated rapid npDC release, thereby inducing neuroprotective immunity at the injury site during the early stage. In addition, pG\/DL@npDC\u2010cryoMNs fostered the formation of a non\u2010typical artificial tertiary lymphoid structure (naTLS) that lacked the complete organized structure of typical TLS yet effectively recruited and engaged immune cells to promote SCI repair. Moreover, pG\/DL@npDC\u2010cryoMNs maintained immunomodulatory activity for up to two weeks, facilitating neuronal regeneration in a mouse model of SCI. Overall, these findings highlight the therapeutic potential of pG\/DL@npDC\u2010cryoMNs in promoting SCI repair by establishing a neuroprotective immune microenvironment.<\/jats:p>","DOI":"10.1002\/smll.202513088","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T17:52:16Z","timestamp":1770659536000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hybrid Cryomicroneedles Enhance DC Vaccine Efficacy and Function as Non\u2010Typical Artificial Tertiary Lymphoid Structures to Provide Neuroprotective Immunity in Spinal Cord Injury"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5485-4693","authenticated-orcid":false,"given":"Xuefeng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Second Affiliated Hospital of Harbin Medical University Harbin Medical University  Harbin P. R. 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