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Herein, we present an innovative, immobilization\u2010free, and label\u2010free paper\u2010based electrochemical fast\u2010flow microfluidic device (eFMD) integrated with near\u2010field communication (NFC) technology for point\u2010of\u2010care detection of MPXV. The platform utilizes a C\u2500C mismatch\u2010mediated Ag\n                    <jats:sup>+<\/jats:sup>\n                    intercalation mechanism, generating distinct current shifts detectable via differential pulse voltammetry (DPV). A microfluidic device with optimized serpentine mixing channels ensures efficient Ag\n                    <jats:sup>+<\/jats:sup>\n                    intercalation without external power sources. The biosensor incorporates laser\u2010converted graphene (LCG) electrodes, offering eco\u2010friendly, scalable, and solvent\u2010free fabrication with rapid prototyping and high design flexibility, ideal for disposable diagnostics. Key assay parameters including buffer type, mismatch probe concentration, AgNO\n                    <jats:sub>3<\/jats:sub>\n                    levels, and detection time were systematically optimized to enhance sensitivity. The biosensor demonstrated excellent analytical performance with a low detection limit down to 1.4 p\n                    <jats:sc>\u043c<\/jats:sc>\n                    for MPXV DNA and significant discrimination against single\u2010base mismatched sequences. Robust performance was further demonstrated in complex matrices, including spiked biological samples and clinically relevant lesion swab specimens, with detection sensitivity down to 1.3 copies mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    and complete concordance with PCR classification. Importantly, the system allows for wireless, on\u2010site readout via smartphones within 35 min, making it suitable for rapid MPXV screening.\n                  <\/jats:p>","DOI":"10.1002\/smll.202513336","type":"journal-article","created":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T20:16:57Z","timestamp":1779653817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Self\u2010Powered Microfluidic Device with Laser\u2010Converted Graphene Electrodes for Immobilization\u2010Free Electrochemical Detection of MPOX Virus DNA via Mismatch\u2010Driven Nanocomplexes"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7622-3541","authenticated-orcid":false,"given":"Wannida","family":"Sapyen","sequence":"first","affiliation":[{"name":"The Institute of Biotechnology and Genetic Engineering Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3330-9771","authenticated-orcid":false,"given":"Nipapan","family":"Ruecha","sequence":"additional","affiliation":[{"name":"Metallurgy and Materials Science Research Institute Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5379-4954","authenticated-orcid":false,"given":"Nopphon","family":"Weeranoppanant","sequence":"additional","affiliation":[{"name":"The Institute of Biotechnology and Genetic Engineering Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Chemical Engineering, Faculty of Engineering Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8397-0750","authenticated-orcid":false,"given":"Chetsada","family":"Khositanon","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering Burapha University Chonburi Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4344-0490","authenticated-orcid":false,"given":"Ekawat","family":"Pasomsub","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Medicine Ramathibodi Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4516-7846","authenticated-orcid":false,"given":"Hongkai","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Hong Kong University of Science and Technology  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8392-5492","authenticated-orcid":false,"given":"Abdulhadee","family":"Yakoh","sequence":"additional","affiliation":[{"name":"The Institute of Biotechnology and Genetic Engineering Chulalongkorn 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