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To achieve this potential, however, selective functionalization of the surface using mild chemical methods that yield spatially and chemically well\u2010defined functional groups is needed. Here, we describe a method to densely and uniformly modify the surface of graphene using a diazonium salt containing a tetrazine moiety, a functional group amenable to bioorthogonal chemistry. The resulting functionalized graphene efficiently undergoes inverse electron demand Diels\u2010Alder reactions with\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010cyclooctene (TCO) coupled compounds, including a small organic molecule and an anti\u2010GFP nanobody. The nanobody decorated graphene readily captures EGFP tagged apoferritin (EGFP apo\u2010Ftn) and by following the binding by total internal fluorescence microscopy, we demonstrate that the functionalized graphene can robustly capture EGFP apo\u2010Ftn molecules at a concentration of 2\u00a0p\n                    <jats:sc>m<\/jats:sc>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/smll.202513723","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T17:07:28Z","timestamp":1774458448000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tetrazine Functionalized Graphene Enables Capture of Ultra\u2010Low Concentrations of Biomacromolecules"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6859-6136","authenticated-orcid":false,"given":"Ravindra K.","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of 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