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The treatment of implant infection is tricky because bacterial biofilms performed on the implants are difficult to remove, and bacteria in the biofilm have high resistance and tolerance to antibiotics. Herein, we report a sustained release depot of a thermosensitive glycoside hydrolase of ELP\u2010DspB, in which dispersin B (DspB) is genetically fused to a thermosensitive elastin\u2010like polypeptide (ELP), to efficiently degrade bacterial biofilms and thus overcome antibiotic resistance. ELP\u2010DspB possesses the antibiofilm bioactivity of DspB to synergize with antibiotics as well as the thermosensitivity of ELP. Moreover, it not only improves the stability of DspB but also diminishes the immunogenicity of DspB. In a mouse model of implant infection by methicillin resistant\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    (MRSE), the thermosensitivity of ELP\u2010DspB enabled the formation of a one\u2010month sustained\u2010release drug reservoir upon topical administration near the MRSE biofilm\u2010infected implant. In combination with antibiotics, a single subcutaneous injection of ELP\u2010DspB efficiently eradicated implant infections without detectable side effects, which was not achieved by DspB. Generally, the ELP fusion is a promising strategy to improve the efficacy of glycoside hydrolases in synergy with antibiotics in eradicating biofilm\u2010related implant infections.\n                  <\/jats:p>","DOI":"10.1002\/smll.202514217","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T03:09:46Z","timestamp":1775012986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Sustained\u2010Release Depot of Thermosensitive Polypeptide Fused Glycosidase Synergizes with Vancomycin to Eradicate Implant Infections"],"prefix":"10.1002","volume":"22","author":[{"given":"Hao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Peking University First Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering 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