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Cortical organoids derived from human\u2010induced pluripotent stem cells provide physiologically relevant models to study such neurotoxic effects. However, accurate imaging of nuclear morphology, a key indicator of cytotoxicity and developmental impairment, remains difficult. Traditional fluorescence\u2010based techniques rely on staining and sectioning, limiting throughput and potentially altering native structures. Herein, we present an ultraviolet photoacoustic microscopy system that targets endogenous nucleic acids at 266\u00a0nm excitation, resulting in a lateral resolution of 278\u00a0nm, which is sufficient to resolve individual nuclei in situ. The system integrates precise z\u2010axis scanning for focal\u2010plane alignment, enabling high\u2010resolution depth\u2010resolved imaging of subvolumes within 3D intact live organoids without physical sectioning. Using EtOH\u2010treated cortical organoids as a model of FASD\u2010associated neurotoxicity, we observed significant reductions in nuclear area, diameter, and circularity by 46.1%, 20.8%, and 6.0%, respectively, indicating structural damage consistent with apoptosis and impaired neurodevelopment. This method is the first to demonstrate label\u2010free nuclear imaging in intact live brain organoids, providing a robust and preparation\u2010free platform for probing disease\u2010relevant phenotypes, accelerating drug screening, and enabling early toxicity assessments in neurodevelopmental disorder models.<\/jats:p>","DOI":"10.1002\/smll.202600048","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T10:51:08Z","timestamp":1783939868000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Label\u2010Free Ultraviolet Photoacoustic Microscopy Enables Nuclear Imaging and Toxicity Assessment in an Intact Brain Organoid"],"prefix":"10.1002","volume":"22","author":[{"given":"Hyunjun","family":"Kye","sequence":"first","affiliation":[{"name":"Department of Biophysics Institute of Quantum Biophysics Sungkyunkwan University  Suwon Republic of Korea"},{"name":"Department of 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