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Conventional live\u2010cell PPI detection techniques, which rely on the delivery of high\u2010molecular\u2010weight fluorescent protein\u2010tagged plasmids into large cell populations, are constrained by low delivery efficiency and inadequate single\u2010cell analytical capability. To address these, we report \u201cPixar,\u201d for\n                    <jats:styled-content>p<\/jats:styled-content>\n                    rotein\n                    <jats:styled-content>i<\/jats:styled-content>\n                    nteraction e\n                    <jats:styled-content>xa<\/jats:styled-content>\n                    mination and\n                    <jats:styled-content>r<\/jats:styled-content>\n                    eal\u2010time behavior monitoring. 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The Pixar system establishes a versatile strategy for exploring protein function and cellular behavioral phenotypes with single\u2010cell precision.\n                  <\/jats:p>","DOI":"10.1002\/smll.73336","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T10:28:48Z","timestamp":1775557728000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Throughput Single\u2010Cell Biochip System for Functional Interrogation of Protein Interactions in Living Cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Feng","family":"Liu","sequence":"first","affiliation":[{"name":"School of Engineering Medicine Beihang University  Beijing China"},{"name":"Qingdao Research Institute Beihang University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbao","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering 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