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Current accurate analysis relating to NfL relies on state\u2010of\u2010the\u2010art technologies such as the single\u2010molecule array (Simoa) and immunoprecipitation\u2010mass spectrometry (IP\u2010MS), which require complicated machinery, skilled operational personnel, and well\u2010equipped laboratories. Herein, we demonstrate a robust on\u2010chip graphene field\u2010effect transistor (GFET) biosensing platform for the ultrasensitive detection of NfL. This work utilizes smaller antibody fragments F(ab\u2019)\n                    <jats:sub>2<\/jats:sub>\n                    to mitigate Debye screening and enhance sensing performance, alongside quantitative characterization of 1\u2010pyrenebutyric acid N\u2010hydroxysuccinimide ester (PBASE) surface density to support controlled antibody immobilization. Compared with whole antibody\u2010based GFETs, this F(ab\u2019)\n                    <jats:sub>2<\/jats:sub>\n                    \u2010modified GFET platform is shown to achieve a 114% increase in sensitivity, a fivefold improvement in the limit\u2010of\u2010detection (LoD) down to 0.18\u00a0pg\/mL, and a wide dynamic detection range from 0.18 to 1500\u00a0pg\/mL, together with good selectivity, stability, and reproducibility. This biosensing platform is validated against Simoa technology for the detection of NfL in clinical plasma samples, yielding a high correlation coefficient of 0.99. These results demonstrate the potential of GFETs for point\u2010of\u2010care diagnosis and the monitoring of neurological diseases in frontline clinical settings, outperforming conventional immunoassays and approaching Simoa sensitivity.\n                  <\/jats:p>","DOI":"10.1002\/smll.73928","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T10:31:23Z","timestamp":1779791483000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ultrasensitive Detection of Neurofilament Light in Plasma Using F(Ab\u2019)\n                    <sub>2<\/sub>\n                    \u2010Modified Graphene Field\u2010Effect Biosensor"],"prefix":"10.1002","volume":"22","author":[{"given":"Selvinaz Burcu","family":"Kizilates","sequence":"first","affiliation":[{"name":"Institute For Materials Discovery University College London  London 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