{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:08:25Z","timestamp":1784887705258,"version":"3.55.0"},"reference-count":67,"publisher":"Wiley","issue":"41","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1600503"],"award-info":[{"award-number":["2023YFD1600503"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Thiram (TMTD), a widely used dithiocarbamate fungicide, provokes oxidative hepatotoxicity, yet practical strategies for its detection and immunoprotection remain scarce. Here we develop an antibody\u2010enabled framework integrating detection, therapy, and prevention against TMTD. In the murine model, we characterized the dose\u2010dependent hepatic injury induced by TMTD. Guided by rational C\n                    <jats:sub>3<\/jats:sub>\n                    \/C\n                    <jats:sub>7<\/jats:sub>\n                    hapten design, we then generate a monoclonal antibody with high affinity. Building on this reagent, we create a portable immunodetection platform that couples Au nanoflowers with an Au\/Ir@Zn\/Cu\u2010MOF probe, achieving an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 0.246\u00a0\u00b5g\/mL. To evaluate protective efficacy, we demonstrated that passive immunization conferred a clear time\u2010dependent therapeutic benefit: mortality decreased from 37.5% in the TMTD\u2010poisoned group to 0% with treatment at 15 or 30\u00a0min and to 12.5% with treatment at 60\u00a0min, while improving body weight, liver function, and oxidative\u2010stress markers. Complementarily, active immunization with TMTD\u2010BSA elicited high antibody titers and conferred complete survival with graded histopathological protection. Together, this work offers an integrated detection\u2010to\u2010protection strategy that effectively bridges immunodetection with immune protection against TMTD\u2010induced toxicity.\n                  <\/jats:p>","DOI":"10.1002\/smll.74084","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:31:57Z","timestamp":1780569117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering a High\u2010Affinity Monoclonal Antibody for Thiram Immunoassays and Protection"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6640-8282","authenticated-orcid":false,"given":"Linfang","family":"Lu","sequence":"first","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Li","sequence":"additional","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshani","family":"Madushika","sequence":"additional","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0812-0037","authenticated-orcid":false,"given":"Sumei","family":"Ling","sequence":"additional","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihua","family":"Wang","sequence":"additional","affiliation":[{"name":"The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins School of Life Sciences, and School of Animal Science Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10102\u2010012\u20100005\u20106"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3693"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4844"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.766725"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.106010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.128808"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.114225"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161\u2010813X(03)00013\u20105"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1964.03060310015003"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics11100851"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010022\u201022731\u20104"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039\u20109140(90)80194\u2010K"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2013.05.020"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2020.08.038"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114199"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.132223"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn100760f"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007218\u20101"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.56471"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-024-00342-2"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4LC01075B"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2022.130615"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.127746"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1230444"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201808240"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010025\u201000869\u2010w"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010025\u201001185\u2010y"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.7b00288"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201043126\u20100"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.11.021"},{"key":"e_1_2_10_32_1","first-page":"3389","article-title":"Effects of Thiram Exposure on Liver Metabolism of Chickens","volume":"10","author":"Wang M.","year":"2023","journal-title":"Frontiers in Veterinary Science"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf101152m"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.133977"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2022.340699"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b03834"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10123106"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21394-x"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.135332"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.135428"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1088\/2050\u20106120\/ad3891"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198211253072201"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapsychiatry.2022.4020"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2021.1927725"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14112290"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm200461m"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.118.253674"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b04872"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fsufs.2024.1490750"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2017.4700"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2022.133928"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000378g"},{"key":"e_1_2_10_53_1","article-title":"Gold Nanoparticle\u2010Based Paper Sensor for Simultaneous Detection of 11 Benzimidazoles by One Monoclonal Antibody","volume":"14","author":"Guo L.","year":"2017","journal-title":"Small"},{"key":"e_1_2_10_54_1","article-title":"Generation of Murine Monoclonal Antibodies by Hybridoma Technology","volume":"119","author":"Hanack K.","year":"2017","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.04.049"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.141131"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2023.110113"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.139372"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.140960"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.02.049"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c00126"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.115033"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010017\u20100714\u2010y"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.137847"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.136419"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c00838"},{"key":"e_1_2_10_67_1","first-page":"817","article-title":"The Past and Present of Serum Aminotransferases and the Future of Liver Injury Biomarkers","volume":"11","author":"McGill M. R.","year":"2016","journal-title":"EXCLI Journal"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.74084","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.74084","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.74084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T09:46:39Z","timestamp":1784886399000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.74084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":67,"journal-issue":{"issue":"41","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/smll.74084"],"URL":"https:\/\/doi.org\/10.1002\/smll.74084","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]},"assertion":[{"value":"2025-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e74084"}}