{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:46:22Z","timestamp":1792082782893,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"48","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172307"],"award-info":[{"award-number":["32172307"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172175"],"award-info":[{"award-number":["32172175"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["JUSRP202404014"],"award-info":[{"award-number":["JUSRP202404014"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Invasive fungal infections, particularly those caused by\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    , have emerged as a significant public health threat. Accurate and timely recognition of\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    is essential for effective diagnosis and treatment. This study introduces a micro\u2010 and nanoscale\u2010enabled diagnostic strategy that integrates porous hydrogel\u2010mediated one\u2010step aptamer selection, nanostructured magnetic capture, and CRISPR\u2010based assay for sensitive\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    detection. First, a diffusion\u2010binding screening method in porous PEG hydrogels was developed to select high affinity aptamers that can recognize the mannoprotein from\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    cell walls. Then, the selected aptamers were conjugated to magnetic beads (MBs) and further enhanced by rolling circle amplification (RCA), forming high\u2010density nanostructured complexes (Apt\u2010RCA@MBs) capable of capturing fungal yeast cells with &gt;90% efficiency. Finally, coupling this nanostructured capture interface with a one\u2010pot isothermal CRISPR\u2010RPA quantification (OICRQ) assay enabled rapid and sensitive detection of\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    in complex clinical samples and food samples, including blood, urine, saliva, wine, and grape juice. This work demonstrates how rational hydrogel microenvironment design and nanostructure\u2010assisted molecular recognition can enhance bioanalytical performance, providing a versatile platform for fungal pathogen detection with potential applications in clinical diagnostics and food safety.\n                  <\/jats:p>","DOI":"10.1002\/smll.74416","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T12:57:23Z","timestamp":1783083443000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Porous Hydrogel\u2010Mediated One\u2010Step Selection of Mannoprotein\u2010Targeted Aptamers for Early Diagnosis of Invasive\n                    <i>Saccharomyces cerevisiae<\/i>\n                    Infections"],"prefix":"10.1002","volume":"22","author":[{"given":"Zhihao","family":"Yao","sequence":"first","affiliation":[{"name":"State Key Laboratory For Quality and Safety of Agro\u2010products Institute of Agro\u2010product Safety and Nutrition Zhejiang Academy of Agricultural Sciences  Hangzhou China"},{"name":"The Key Laboratory of Industrial Biotechnology State Key Laboratory of Food Science and Resources Ministry of Education School of Biotechnology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyu","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory For Quality and Safety of Agro\u2010products Institute of Agro\u2010product Safety and Nutrition Zhejiang Academy of Agricultural Sciences  Hangzhou China"},{"name":"Key Laboratory of Information Traceability For Agricultural Products Ministry of Agriculture and Rural Affairs  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory For Quality and Safety of Agro\u2010products Institute of Agro\u2010product Safety and Nutrition Zhejiang Academy of Agricultural Sciences  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0192-2352","authenticated-orcid":false,"given":"Steven","family":"Suryoprabowo","sequence":"additional","affiliation":[{"name":"Food Technology Department Faculty of Engineering Bina Nusantara University  Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Nie","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Industrial Biotechnology State Key Laboratory of Food Science and Resources Ministry of Education School of Biotechnology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6756-196X","authenticated-orcid":false,"given":"Qun","family":"Wu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Industrial Biotechnology State Key Laboratory of Food Science and Resources Ministry of Education School of Biotechnology Jiangnan University  Wuxi Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8088-0145","authenticated-orcid":false,"given":"Liu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory For Quality and Safety of Agro\u2010products Institute of Agro\u2010product Safety and Nutrition Zhejiang Academy of Agricultural Sciences  Hangzhou China"},{"name":"Key Laboratory of Information Traceability For Agricultural Products Ministry of Agriculture and Rural Affairs  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201005856\u20105"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201064001\u20100"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2019.03.007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010024\u201001662\u20105"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/497832"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c09916"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.05.021"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2022.2147899"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11042\u2010024\u201019649\u2010z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201040479\u20104"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2025.02.009"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1109\/JSEN.2021.3072387"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.448729"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2024.2404041"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5c03586"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.11.6823\u20106830.2005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2024.105290"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544\u2010021\u201000582\u2010z"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2024.103276"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.171154"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c17041"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c05700"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117619"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf219"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c03061"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.161848"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202407799"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201053693\u20105"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941\u2010023\u201000570\u20106"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw864"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116996"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010025\u201001868\u20106"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010023\u201001973\u20108"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202504107"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115354"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42114\u2010025\u201001319\u20109"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118758"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.136425"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202403371"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117627"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.3c02485"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202501269"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.74416","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.74416","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.74416","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:50:31Z","timestamp":1792079431000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.74416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,3]]},"references-count":42,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/smll.74416"],"URL":"https:\/\/doi.org\/10.1002\/smll.74416","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,3]]},"assertion":[{"value":"2026-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e74416"}}