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However, conventional tissue engineering strategies often face challenges in fabricating tissues simultaneously incorporating controlled cellular and extracellular matrix (ECM) architectures. Here, we introduce Acoustic Tweezers\u2010Assisted Biomaterial Molding in Petri Dishes (TAMP), enabling tissue fabrication with controlled cellular and ECM architectures in Petri dishes, specifically, producing constructs with customized ECM geometries and various internal cellular architectures, including parallel elongated cell bundles, arrays of interconnected cell spheroids, and lattice\u2010like cellular networks. TAMP leverages a portable acoustic array that delivers standing waves into a Petri dish or a polydimethylsiloxane (PDMS) mold, thereby enabling a unique \u201cdual\u2010control\u201d mechanism. The acoustic field arranges internal cells into parallel line\u2010like and lattice\u2010like patterns, while the PDMS mold defines the overall ECM geometry. Our approach was demonstrated by arranging micro\u2010objects into various patterns within different\u2010shaped molds and fabricating glioma tissues with various unique internal cellular architectures, including parallel elongated glioma tissue bundles, lattice\u2010like glioma tissue networks, and chains of interconnected glioma spheroids. We anticipate the TAMP technique will lead to portable, easy\u2010to\u2010operate tools for engineering tissues with controlled cellular and ECM architectures for biomedical research, disease modeling, and drug testing.<\/jats:p>","DOI":"10.1002\/smll.74599","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T04:10:33Z","timestamp":1784520633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acoustic Tweezers\u2010Assisted Biomaterial Molding in Petri Dishes (TAMP) for Engineering Glioma Tissues With Controlled Cellular and Extracellular Matrix Architectures"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9306-2608","authenticated-orcid":false,"given":"Yingshan","family":"Du","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Virginia Polytechnic Institute and State University  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9432-3776","authenticated-orcid":false,"given":"Jiali","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Virginia Polytechnic Institute and State University  Blacksburg Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7173-1420","authenticated-orcid":false,"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Weifang People's Hospital Shandong Second Medical University  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4690-6337","authenticated-orcid":false,"given":"Julio P.","family":"Arroyo","sequence":"additional","affiliation":[{"name":"Wallace H. 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