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However, current biosensors cannot distinguish between living and dead bacteria, critical in determining the persistence of bacterial contamination. The Meta\u2010Nano\u2010Channel field\u2010effect transistor (MNC FET) is employed. Its design allows the formation of numerous conducting nano\u2010channels each coupled to the sensing area binding events with a different efficiency addressing the challenge of molecular localized gating. The MNC FET is biofunctionalized with a recognition layer composed of\n                    <jats:italic>E. coli<\/jats:italic>\n                    antibodies. Sensing is performed in 0.5\u00a0\u00b5L drops of Lysogeny Broth (ionic strength of 197\u00a0mM, protein\u2010rich background of 200\u00a0mg\/L), providing a complex food environment, and without either sample pre\u2010processing or pre\u2010measurement washing. Specific, label\u2010free, quantitative, and real\u2010time sensing is demonstrated with a limit\u2010of\u2010detection of a single\u2010bacterium and with excellent linearity and sensitivity. Furthermore, the platform's capacity to distinguish living from dead bacteria is validated by comparing the MNC FET response to living\n                    <jats:italic>E. coli<\/jats:italic>\n                    against populations subjected to thermal denaturation, antibiotic treatment, or metabolic inhibition. The MNC FET distinguishes between living and dead\n                    <jats:italic>E. coli<\/jats:italic>\n                    bacteria with a limit\u2010of\u2010detection of a single\u2010bacterium. This method paves the way for biosensors for the discrimination of bacterial viability.\n                  <\/jats:p>","DOI":"10.1002\/smll.74900","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:00:36Z","timestamp":1785229236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discriminating Between Living and Dead Bacteria With a Field\u2010Effect Transistor Based on Nano\u2010Channels"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7249-0422","authenticated-orcid":false,"given":"Bar","family":"Chen","sequence":"first","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Garika","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3596-5921","authenticated-orcid":false,"given":"Shubham","family":"Babbar","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6349-6920","authenticated-orcid":false,"given":"Pooja","family":"Verma","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Cohen","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5090-367X","authenticated-orcid":false,"given":"Kfir","family":"Kattav","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Akselrod","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparnaa Shree","family":"Vijaikumar","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Data Science Research Centre Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7699-3086","authenticated-orcid":false,"given":"Shankar","family":"Bhattarai","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Data Science Research Centre Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherina","family":"Harilal","sequence":"additional","affiliation":[{"name":"Department of Solar Energy and Environmental Physics Swiss Institute for Dryland Environmental and Energy Research J. 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Blaustein Institutes for Desert Research Ben\u2010Gurion University of the Negev  Midreshet Ben\u2010Gurion Israel"},{"name":"The Ilse\u2010Katz Institute for Nanoscale Science and Technology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izhar","family":"Ron","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakov","family":"Roizin","sequence":"additional","affiliation":[{"name":"Tower Semiconductor  Migdal Haemek Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3882-2742","authenticated-orcid":false,"given":"Barak","family":"Akabayov","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Data Science Research Centre Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6893-1720","authenticated-orcid":false,"given":"Gil","family":"Shalev","sequence":"additional","affiliation":[{"name":"School of Electrical and computer Engineering Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"},{"name":"The Ilse\u2010Katz Institute for Nanoscale Science and Technology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"World Health Organization Foodborne Diseases(2026)https:\/\/www.who.int\/news\/item\/04\u201006\u20102026\u2010unsafe\u2010food\u2010causes\u2010866\u2010million\u2010illnesses\u2010and\u20101.5\u2010million\u2010deaths\u2010annually\u2013young\u2010children\u2010at\u2010highest\u2010risk(2026)."},{"key":"e_1_2_7_3_1","unstructured":"World Health Organization Estimating the Burden of Foodborne Diseases(2024) https:\/\/www.who.int\/activities\/estimating\u2010the\u2010burden\u2010of\u2010foodborne\u2010diseases."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(16)31012\u20101"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2019.11.007"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0327-x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.103"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1000664013047"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010024\u201000559\u20109"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-811942-6.00013-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e15482"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01763\u201014"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20101605(99)00072\u20100"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00016\u201010"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02750\u201006"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","unstructured":"K. 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