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Existing fluorescent optofluidic chips achieve high sensitivity but rely on bench\u2010top optics, pumps, and trained personnel. Here, we present a smart, disposable evanescent wave fluorescent optofluidic bio\u2010nanochip (DEFOB) that bridges this performance gap. The DEFOB integrates a gravity\u2010driven, 3D\u2010printed microfluidic chip with a functionalized tapered fiber nanosensor, a compact alignment\u2010free all\u2010fiber optical reader, and a smartphone application. This synergistic design enables ultrasensitive, quantitative detection in both homogeneous and heterogeneous assay formats without the need for external pumps or complex peripherals. We demonstrate the platform's versatility through the laboratory\u2010comparable quantification of sulfamerazine antibiotics via immunoassay and of SARS\u2010CoV\u20102 via an integrated RAA\u2010CRISPR\/Cas12b assay, achieving detection limits of 0.06\u00a0\u00b5g\/L and 0.70 copies\/\u00b5L, respectively. By combining dual\u2010mode operation, high sensitivity, quantitative accuracy, intelligence, plug\u2010and\u2010play functionality, and a low\u2010cost workflow, the DEFOB platform represents a paradigm shift in PON testing, with broad applications in clinical diagnostics, environmental monitoring, and global health security.<\/jats:p>","DOI":"10.1002\/smll.75033","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T10:30:41Z","timestamp":1785839441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Smart, Gravity\u2010Driven, Dual\u2010Mode, Disposable Evanescent Wave Fluorescent Optofluidic Bio\u2010Nanochip for Rapid and Sensitive Point\u2010of\u2010Need Testing of Trace Pollutants"],"prefix":"10.1002","volume":"22","author":[{"given":"Siyan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Chemistry and Life Resources Renmin University of China  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayuan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Life Resources Renmin University of China  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqiang","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Chemistry and Life Resources Renmin University of China  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikan","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Chemistry and Life Resources Renmin University of China  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Economics and Management Ningbo University of Technology  Ningbo People's Republic of 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