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The in\u2010situ polymerization method has the potential to control the encapsulation shell but lacks chemical driving forces to enable shell thickness from nanoscale to micrometer\u2010scale. The micrometer\u2010scale shell can enhance the functionality in cell encapsulation and extend its applications to broader fields. In this work, we utilized the efficient and mild thiol\u2010alkene click reaction to construct a micrometer\u2010scale shell and encapsulate microbial cells into large\u2010sized polymer microspheres. The encapsulated microbial cells are larger than the coexisting emulsion colloids and particulate impurities, thereby achieving \u201cConvert microbial cell detection to polymer particle counting\u201d and providing a new perspective for the application of micrometer\u2010scale artificial cell shells.<\/jats:p>","DOI":"10.1002\/smll.75038","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T09:57:12Z","timestamp":1785751032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Non\u2010Stoichiometric Click Polymerization for Size\u2010Controlled and Mild Encapsulation of Cells"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6513-2643","authenticated-orcid":false,"given":"Yao\u2010Yu","family":"Pan","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering and Health Wuhan Textile University  Wuhan People's Republic of 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