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A key virulence mechanism is the acquisition of iron from the heme group of human host's hemoglobin, initiated by hemoglobin binding to receptors exposed on the bacterial surface. Although this interaction is a promising target for new antimicrobials, quantitative data on hemoglobin capture by single bacteria are lacking. In this work, single\u2010molecule localization microscopy is used to directly quantify the number of hemoglobin molecules captured by individual\n                    <jats:italic>S. aureus<\/jats:italic>\n                    cells and resolve their nanoscale spatial distribution at the sub\u2010cellular level. This quantitative approach provides a methodological framework to monitor, with single\u2010molecule sensitivity, changes in surface density of functional receptors in response to iron limitations. This piece of information, in turn, is critical to define the overall efficacy of antimicrobials targeting bacterial receptors. As proof of this concept, we develop a photo\u2010activated molecular system based on hemoglobin for targeted photodynamic inactivation of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/smll.75276","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:23:43Z","timestamp":1787052223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Imaging of Human Hemoglobin Binding to\n                    <i>Staphylococcus aureus<\/i>\n                    With Single\u2010Molecule Resolution"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8235-765X","authenticated-orcid":false,"given":"Pietro","family":"Delcanale","sequence":"first","affiliation":[{"name":"Dipartimento Di Scienze Matematiche Fisiche e Informatiche Universit\u00e0 di Parma  Parma 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