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However, the reported chemiluminescent scaffolds remain limited, and the development of novel and efficient chemiluminescent scaffolds is critical and urgent. Herein, we synthesized a series of peroxynitrite (ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    )\u2010activated chemiluminescent probes based on a hemicyanine scaffold through molecular engineering, and systematically investigated optical properties as well as their response mechanisms. Upon reaction with ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    , the vinylene units in the hemicyanine scaffold are oxidatively degraded, thereby emitting a chemiluminescent signal in the 400\u2013650\u00a0nm wavelength range. Furthermore, under the defined conditions, it was found that the tissue penetration of ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2010activated chemiluminescence (up to 12\u00a0mm) is deeper than that of 660\u00a0nm\u2010activated afterglow luminescence and the fluorescence of hemicyanine scaffolds. In cell imaging, in addition to sensitive ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    response via chemiluminescence, it can also respond superiorly to ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    through well\u2010separated dual fluorescence channels. In conjunction with satisfactory biocompatibility, the probe showed favorable responsiveness and high sensitivity toward ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    levels in lipopolysaccharide\u2010induced inflammation induced. These findings provide solid and reliable experience for the discovery and construction of novel and versatile chemiluminescent scaffolds.\n                  <\/jats:p>","DOI":"10.1002\/smll.75297","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:39:31Z","timestamp":1787060371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Engineering of Hemicyanine Scaffold for Peroxynitrite\u2010Activated Chemiluminescence Imaging"],"prefix":"10.1002","volume":"22","author":[{"given":"Mingyue","family":"Wu","sequence":"first","affiliation":[{"name":"College &amp; Hospital of Stomatology Anhui Provincial Key Laboratory of Oral Diseases Research Anhui Medical University  Hefei China"},{"name":"School of Pharmacy Anhui Medical University Inflammation and Immune Mediated Diseases Laboratory of Anhui Province  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunchun","family":"Zha","sequence":"additional","affiliation":[{"name":"College &amp; Hospital of Stomatology Anhui Provincial Key Laboratory of Oral Diseases Research Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College &amp; Hospital of Stomatology Anhui Provincial Key Laboratory of Oral Diseases Research Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongtai","family":"Xu","sequence":"additional","affiliation":[{"name":"College &amp; Hospital of Stomatology Anhui Provincial Key Laboratory of Oral Diseases Research Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second 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