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Dense poly(ethylene glycol) methyl ether methacrylate (PEGMEMA) brush films synthesized by surface\u2010initiated atom transfer radical polymerization yield a linear growth rate of 0.83 nm min\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    and thicknesses up to 120 nm. Quartz crystal microbalance with dissipation monitoring confirms suppression of non\u2010specific protein adsorption from fetal bovine serum. Terminal azide functionalization followed by strain\u2010promoted copper\u2010free click chemistry enables immobilization of linear Arg\u2010Gly\u2010Asp (linRGD) and cyclic (cRGD) peptides at independently tunable densities, a design space of brush thickness, peptide density, and RGD conformation not previously achieved in microfluidics. Integration into polydimethylsiloxane microfluidic channels with a progressive force ramp (0\u201325 dynes cm\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>2<\/jats:sup>\n                    ) achieves selective displacement of A375 melanoma from A2780 ovarian cancer cells with 88\u00a0\u00b1\u00a015% recovery and &gt;93% viability. Differential focal adhesion maturation underlies separation: A375 cells express 29.7% lower \u03b15\u03b21 integrin density and form nascent adhesions retracting at 120 min (actin falls 37%), whereas A2780 cells develop mature focal adhesions with 92% and 125% increases in talin and actin. This platform transduces integrin profiles and cytoskeletal competence into measurable adhesion phenotypes, enabling precise, gentle label\u2010free cell separation.\n                  <\/jats:p>","DOI":"10.1002\/smll.75311","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:50:55Z","timestamp":1787129455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Peptide Terminated Polymer Brush Nanomaterials for Label\u2010Free Separation of Cancer Cells Based on Relative Adhesion"],"prefix":"10.1002","volume":"22","author":[{"given":"Stephen","family":"Horan","sequence":"first","affiliation":[{"name":"School of Chemistry and Conway Institute for Biomolecular and Biomedical Research University College Dublin  Dublin Republic of Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Wdowicz","sequence":"additional","affiliation":[{"name":"School of Chemistry and Conway Institute for Biomolecular and Biomedical Research University College Dublin  Dublin Republic of Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5109-6298","authenticated-orcid":false,"given":"Nathan","family":"Feely","sequence":"additional","affiliation":[{"name":"School of Chemistry and Conway Institute for Biomolecular and Biomedical Research University College Dublin  Dublin Republic of Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chemistry and Conway Institute for Biomolecular and Biomedical Research University College Dublin  Dublin Republic of Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5773-6647","authenticated-orcid":false,"given":"Oya","family":"Tagit","sequence":"additional","affiliation":[{"name":"Magnostics Ltd, Co.  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