{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:12:25Z","timestamp":1788437545026,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471986"],"award-info":[{"award-number":["32471986"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["323B2053"],"award-info":[{"award-number":["323B2053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["226202400169"],"award-info":[{"award-number":["226202400169"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2024FZZX02\u201001\u201008"],"award-info":[{"award-number":["2024FZZX02\u201001\u201008"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In situ monitoring of plant responses to stress is one of the most challenging aspects of precision agriculture, and the dynamic control of crop growth according to fluctuating environmental factors. Although fluorescence imaging provides a nondestructive approach for monitoring stress\u2010related biomarkers, its performance is often hindered by the low abundance of endogenous signaling molecules and strong tissue autofluorescence. Here, we report a microneedle\u2010integrated sensing platform that enables sensitive detection of endogenous hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) in living plants. The platform incorporates a second near\u2010infrared fluorescent nanoprobe composed of Er\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010doped lanthanide nanoparticles emitting at 1550\u00a0nm and Mo\u2010doped polymetallic oxomolybdates serving as the H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010responsive unit. Embedding the nanoprobe into custom\u2010fabricated microneedles allows precise positioning on plant midribs for continuous monitoring of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    dynamics. Under stress conditions, the system successfully visualized spatiotemporal fluctuations of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    in living tomato, spinach, and tobacco plants. This work establishes a strategy for early stress diagnosis and developing universal plant health monitoring technologies.\n                  <\/jats:p>","DOI":"10.1002\/smll.75475","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:13:50Z","timestamp":1788434030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Situ Monitoring of Stress\u2010Induced Hydrogen Peroxide in Plants Using NIR\u2010II Fluorescent Microneedles"],"prefix":"10.1002","author":[{"given":"Shengchun","family":"Sun","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxing","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Tong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silicon and Advanced Semiconductor Materials, Institute For Composites Science Innovation, School of Materials Science and Engineering Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8213-6304","authenticated-orcid":false,"given":"Renren","family":"Deng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silicon and Advanced Semiconductor Materials, Institute For Composites Science Innovation, School of Materials Science and Engineering Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6604-360X","authenticated-orcid":false,"given":"Yibin","family":"Ying","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"},{"name":"ZJU\u2010Hangzhou Global Scientific and Technological Innovation Center  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8669-7598","authenticated-orcid":false,"given":"Yixian","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province  Hangzhou China"},{"name":"ZJU\u2010Hangzhou Global Scientific and Technological Innovation Center  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/s43016-021-00322-9","article-title":"A Meta\u2010Analysis of Projected Global Food Demand and Population at Risk of Hunger for the Period 2010\u20132050","volume":"2","author":"Dijk M.","year":"2021","journal-title":"Nature Food"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201044588\u2010y"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748\u20109326\/10\/1\/014010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101679\u20100"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.3390\/rs13132585","article-title":"Advances in IoT and Smart Sensors for Remote Sensing and Agriculture Applications","volume":"13","author":"Ullo S. L.","year":"2021","journal-title":"Remote Sensing"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10531\u2010023\u201002658\u2010z"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010022\u201000499\u20102"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14060617"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.003954"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00230"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.3390\/ijms19092812","article-title":"Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks","volume":"19","author":"\u010cern\u00fd M.","year":"2018","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ert375"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477\u2010022\u201001256\u20101"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.aam9514","article-title":"Coordinating the Overall Stomatal Response of Plants Rapid Leaf\u2010to\u2010Leaf Communication during Light Stress","volume":"11","author":"Amith S. I. Z.","year":"2018","journal-title":"Science Signaling"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2018.10.006"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298\u2010025\u201001673\u20104"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erl004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijpb14030043"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4236\/ajac.2014.511081"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2023.11.021"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.07.039"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201060182\u2010w"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477\u2010020\u20100632\u20104"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201802889"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202207651","article-title":"Self\u2010Illuminating NIR\u2010II Chemiluminescence Nanosensor for in Vivo Tracking H2O2 Fluctuation","volume":"10","author":"Zhang S.","year":"2023","journal-title":"Advanced Science"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00644"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566\u2010025\u201001736\u20108"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100262\u20104"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201046727\u20105"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.9b05159"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41377\u2010024\u201001518\u2010x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116114"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2025.118409"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116131"},{"key":"e_1_2_7_36_1","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202402024","article-title":"Rapid and in\u2010Field Sensing of Hydrogen Peroxide in Plant by Hydrogel Microneedle Patch","volume":"20","author":"Wu X.","year":"2024","journal-title":"Small"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b00193"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010025\u201001923\u20102"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c04356"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b05148"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c00548"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202001544"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202301683"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000917\u20106"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.optmat.2015.11.004"},{"key":"e_1_2_7_46_1","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1364\/OL.33.002548","article-title":"Enhanced Efficiency of Er:Yb:Ce:NaGd(WO4)2 Laser at 1.5\u20131.6 \u039cm by the Introduction of High\u2010Doping Ce3+ Ions","volume":"33","author":"Jianhua Huang Y. C.","year":"2008","journal-title":"Optics Letters"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1109\/3.541688"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2nj41031a"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201704620"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201903551"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.09.017"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.15.01237"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2022.11.048"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.772649"},{"key":"e_1_2_7_55_1","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1093\/emboj\/16.6.1137","article-title":"Transgenic Tobacco Expressing a Foreign Calmodulin Gene Shows an Enhanced Production of Active Oxygen Species","volume":"16","author":"Scott S. H. O.","year":"1997","journal-title":"The EMBO Journal"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477\u2010022\u201001252\u20105"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010021\u201000929\u20105"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000448"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.120642"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ert275"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02485"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab157"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.04.028"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14685"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aea4466"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2100899"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2009.03.010"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.75475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.75475","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.75475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:13:53Z","timestamp":1788434033000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.75475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":66,"alternative-id":["10.1002\/smll.75475"],"URL":"https:\/\/doi.org\/10.1002\/smll.75475","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]},"assertion":[{"value":"2026-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e75475"}}