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However, DNA polymerases\u2019 intrinsic polymerizing\/exonucleolytic activities cause nonspecific extension\/degradation of hairpins, restricting the combination of these two reactions to stepwise operations with limited efficiency. Herein, we report a guanine\u2010locked DNA circuit resisting polymerase\u2010mediated side reactions, enabling homogeneous coupling of catalytic hairpin assembly and dCTP\u2010free rolling circle amplification. Modified onto magnetic nanoparticles for optomagnetic biosensing, this platform unifies target recognition, amplification, and real\u2010time transduction in a one\u2010pot isothermal format, achieving a detection limit of 9 aM with a wide dynamic range in 1\u00a0h for\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    DNA. The biosensor discriminates\n                    <jats:italic>Botrytis<\/jats:italic>\n                    species and correlates strongly with qPCR (\n                    <jats:italic>r<\/jats:italic>\n                    = \u22120.986) for infected rose petal samples. This work resolves the incompatibility between hairpin cascades and DNA polymerases, providing a generalizable strategy for crosstalk\u2010free homogeneous nucleic acid circuits.\n                  <\/jats:p>","DOI":"10.1002\/smll.75508","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:04:21Z","timestamp":1787814261000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Guanine\u2010Locked DNA Circuit Drives Homogeneous Coupling of Catalytic Hairpin Assembly and dCTP\u2010Free Rolling Circle Amplification"],"prefix":"10.1002","author":[{"given":"Yuan","family":"Fang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemo\/Biosensing and Chemometrics Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation College of Biology Hunan University  Changsha China"},{"name":"Department of Biomedical Engineering Xiangya School of Basic 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