{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:08:08Z","timestamp":1788851288557,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Enzymatic depolymerization of plastics offers a sustainable route to polymer recycling, yet the mechanism of action of poly(ethylene terephthalate) (PET) hydrolases at solid polymer interfaces remains poorly understood. In particular, it is still unclear whether individual enzymes perform isolated cleavage events or catalyze multiple successive reactions during a single adsorption event. Here, we employ fluorogenic probe\u2010doped PET nanoparticles together with a leaf\u2010branch compost cutinase variant (LCC\n                    <jats:sup>ICCG<\/jats:sup>\n                    ) to visualize interfacial PET hydrolysis at the single\u2010molecule level and reveal the dynamic principles governing interfacial catalysis. Fluorescence trajectories reveal multi\u2010burst events with short intervals, consistent with semi\u2010processive hydrolysis during a single enzyme adsorption event. Based on the quantitative correspondence between the experimentally observed fraction of multi\u2010burst events and the simulated nearest\u2010neighbor distance distribution, we estimate an apparent effective reaction distance of approximately 2\u00a0nm for individual enzymes. We further show that burst frequency exhibits a nonmonotonic temperature dependence governed by the surface\u2010chain mobility near the glass transition, whereas the overall PET degradation rate increases continuously. Our findings provide a quantitative framework linking enzyme dynamics, the local reaction environment, and polymer interfacial physics, providing a basis for engineering more efficient PET hydrolases.\n                  <\/jats:p>","DOI":"10.1002\/smll.75670","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:24:28Z","timestamp":1788848668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Molecule Detection of Enzymatic PET Hydrolysis Using Fluorogenic Probe\u2010Doped PET Nanoparticles"],"prefix":"10.1002","author":[{"given":"Asuka","family":"Mouri","sequence":"first","affiliation":[{"name":"Department of Chemistry Graduate School of Science Kobe University  Kobe 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