{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:06:50Z","timestamp":1790006810327,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22407002"],"award-info":[{"award-number":["22407002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2024AH050714"],"award-info":[{"award-number":["2024AH050714"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2024AH050706"],"award-info":[{"award-number":["2024AH050706"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2023AH050551"],"award-info":[{"award-number":["2023AH050551"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Routing chemically distinct small\u2010molecule aptamers into a common nuclease trans\u2010cleavage pathway is constrained by the target\u2010specific output handles of conventional structure\u2010switching aptamers (SSAs). Shared\u2010output aptamer\u2010gated DNA nanodevices are designed so that recognition cores control release of the same short trigger DNA (tDNA). The released tDNA hybridizes with a common guide RNA and activates NbaSPARDA, a short prokaryotic Argonaute\u2010associated nuclease complex, for reporter trans\u2010cleavage. Analyses across two sequence scaffolds reveal sequence\u2010dependent activation and identify a practical 14\u201316\u00a0nt range for the primary scaffold. Native PAGE supports target\u2010induced tDNA release. Under matched conditions, NbaSPARDA produces lower target\u2010free signals than LbCas12a for the compact SSA configurations tested. Adenosine\u2010gated devices achieve detection limits of 0.28 and 0.40\u00a0\u00b5M in buffer, while Ade\u2010SSA14 supports matrix\u2010spiked relative readout of pentostatin\u2010induced adenosine\u2010related changes in processed HeLa lysate filtrates. A mefloquine aptamer reconfigured with the same 16\u00a0nt output achieves detection limits of 0.018\u00a0\u00b5M in buffer and 0.061\u00a0\u00b5M by matrix\u2010matched calibration in an acetonitrile\u2010processed blank\u2010serum extract. Serum samples spiked before processing give recoveries of 107 \u00b1 19% and 101 \u00b1 7.5%. An exploratory estradiol construct accesses the output channel. Together, these results establish a shared\u2010output interface for programming NbaSPARDA trans\u2010cleavage in metabolite and drug analysis.<\/jats:p>","DOI":"10.1002\/smll.75876","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:34:49Z","timestamp":1790004889000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Shared\u2010Output Aptamer\u2010Gated DNA Nanodevices Program NbaSPARDA Trans\u2010Cleavage for Metabolite and Drug Sensing"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-6136","authenticated-orcid":false,"given":"Yi","family":"Du","sequence":"first","affiliation":[{"name":"School of Life Sciences Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyu","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meimei","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0490-1677","authenticated-orcid":false,"given":"Pengfei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine the First Affiliated Hospital of Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6563-1626","authenticated-orcid":false,"given":"Hongyu","family":"Bao","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9655-1047","authenticated-orcid":false,"given":"Pai","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"4961","DOI":"10.1039\/D3SC00439B","article-title":"Aptamers 101: Aptamer Discovery and In Vitro Applications in Biosensors and Separations","volume":"14","author":"Yang L. 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