{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:51:41Z","timestamp":1791204701248,"version":"4.2.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Precisely integrating MOF powders into patterned electrochemical devices remains a processing challenge, as conventional methods often require masks, manual alignment, or poorly scalable steps and can lead to non\u2010selective deposition and inefficient material use. While electrophoretic and dielectrophoretic deposition methods for particulate materials and GOx@ZIF\u20108\/Prussian Blue (PB) glucose biosensors have been reported independently, low\u2010volume localization of enzyme\u2010loaded MOFs directly onto active microelectrode transducer regions remains challenging. Here, we develop a mask\u2010free, DC\u2010field\u2010assisted microdroplet strategy that selectively localizes GOx@ZIF\u20108 onto PB\u2010modified interdigitated electrodes (PB@IDEs). The approach uses only 12\u00a0\u00b5L of GOx@ZIF\u20108 suspension and, unlike random drop\u2010casting, preferentially confines the biocatalytic MOF to the PB transduction interface, minimizing inactive deposition and improving enzyme utilization. The resulting Nafion\/GOx@ZIF\u20108\/PB@IDE biosensor exhibits reproducible spatial localization and approximately threefold higher sensitivity, together with a lower limit of detection, than an otherwise identical drop\u2010cast electrode. The sensor shows a linear amperometric response over 24\u2013175\u00a0\u00b5M glucose concentration, overlapping concentrations relevant to sweat glucose analysis. These results demonstrate that electro\u2010addressable placement of enzyme@MOF particles can improve reaction\u2010readout coupling while reducing biocatalyst consumption.<\/jats:p>","DOI":"10.1002\/smll.75917","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:02:22Z","timestamp":1791201742000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mask\u2010Free Electro\u2010Addressable Localization of GOx@ZIF\u20108 on Prussian Blue Microelectrodes for Glucose Biosensing"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9632-6723","authenticated-orcid":false,"given":"Wenyi","family":"Zeng","sequence":"first","affiliation":[{"name":"Institute of Inorganic Chemistry Faculty of Chemistry University of Vienna  Vienna Austria"},{"name":"Vienna Doctoral School in Chemistry (DoSChem) University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3731-7027","authenticated-orcid":false,"given":"Monnaya","family":"Chalermnon","sequence":"additional","affiliation":[{"name":"Institute of Inorganic Chemistry Faculty of Chemistry University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6276-8875","authenticated-orcid":false,"given":"Flora","family":"Sch\u00f6fbeck","sequence":"additional","affiliation":[{"name":"Vienna Doctoral School in Chemistry (DoSChem) University of Vienna  Vienna Austria"},{"name":"Institute of Functional Materials and Catalysis Faculty of Chemistry University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5391-7232","authenticated-orcid":false,"given":"Tristan Tsai Yuan","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Materials Research and Engineering (IMRE) Agency for Science, Technology and Research (A*STAR)  Singapore Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8020-1720","authenticated-orcid":false,"given":"Jason Y. 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