{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:57:26Z","timestamp":1790528246408,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572505"],"award-info":[{"award-number":["32572505"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sunflower meal is an abundant but underutilized plant protein source. Recent studies have demonstrated that its amyloid fibrils exhibit exceptional functional properties, yet the atomic\u2010level structural basis remains unknown. The first cryo\u2011electron microscopy (cryo\u2011EM) structures of sunflower amyloid fibrils prepared under acidic heating conditions are presented. Two distinct polymorphs (PM1 and PM2) are resolved at 3.05 and 3.18 \u00c5, respectively. They differ markedly in both helical half\u2010pitch (389 \u00c5 for PM1 vs. 1087 \u00c5 for PM2) and handedness (left\u2010handed for PM1 vs. right\u2010handed for PM2). High\u2010resolution atomic modeling reveals that they adopt distinct assembly modes. PM1 is assembled from the acidic (Leu94\u2013Phe115) and basic (Val333\u2013Phe344) subunits of 11S globulin, covalently linked by an inter\u2010subunit disulfide bond (Cys111\u2013Cys338); whereas PM2 is formed by an acidic\u2010subunit segment (Asn30\u2013Val68) and stabilized by an intra\u2010subunit disulfide bond (Cys32\u2013Cys65). Comparison of the PM1 structure with the apricot globulin fibril reveals divergent assembly strategies, most strikingly an opposite orientation of the basic subunit relative to the acidic fragment despite high sequence homology. These structural insights provide a molecular foundation for understanding and engineering sunflower amyloid fibrils for diverse applications in food, agriculture, and nanotechnology.<\/jats:p>","DOI":"10.1002\/smll.75987","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:51:20Z","timestamp":1790527880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cryo\u2010EM Structures of Sunflower Amyloid Fibrils Reveal Two Distinct Disulfide\u2011Linked Assembly Modes"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3177-1364","authenticated-orcid":false,"given":"Saiya","family":"Li","sequence":"first","affiliation":[{"name":"Department of Food Science &amp; Engineering School of Agriculture &amp; Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-6230","authenticated-orcid":false,"given":"Kefan","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Engineering School of Agriculture &amp; Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangjian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Engineering School of Agriculture &amp; Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3411-7648","authenticated-orcid":false,"given":"Yapeng","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Engineering School of Agriculture &amp; Biology Shanghai Jiao Tong University  Shanghai China"},{"name":"School of Health Science &amp; Engineering University of Shanghai for Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Cao","sequence":"additional","affiliation":[{"name":"Bio\u2010X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders Ministry of Education Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7039-5445","authenticated-orcid":false,"given":"Yiping","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Engineering School of Agriculture &amp; Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"USDA. 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