{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T07:38:06Z","timestamp":1792568286299,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LZ26B050002"],"award-info":[{"award-number":["LZ26B050002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tobacco crown rot caused by\n                    <jats:italic>Fusarium oxysporum<\/jats:italic>\n                    is difficult to control because the pathogen persists in soil and disrupts both plant physiology and rhizosphere function. Herein, we developed a multifunctional bacteria\u2010nanozyme biohybrid by immobilizing copper\u2010piceatannol\u2010functionalized mesoporous silica nanoparticles on\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    (BS@MCP). The composite retained bacterial viability while exhibiting peroxidase\u2010like catalytic activity and cuproptosis\u2010like antifungal effects. BS@MCP showed broad\u2010spectrum antimicrobial activity against\n                    <jats:italic>F. oxysporum<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and carbapenem\u2010resistant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , with H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    further enhancing microbial inhibition. Mechanistic analyses indicated that BS@MCP disrupted fungal membrane integrity, promoted ROS accumulation and lipid peroxidation, increased intracellular copper content, impaired respiratory\u2010chain activity, depleted ATP and induced pyruvate accumulation. Antioxidant and copper\u2010chelation rescue experiments further supported the cooperative involvement of oxidative damage and copper\u2010dependent cell death. In non\u2010sterile soil, BS@MCP\/H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    reduced the disease index from 91.0% in infected plants to 24.7%, while increasing plant height by 63.5%, root length by 120.7%, fresh biomass by 99.7%, and dry biomass by 225.6%. The treatment enhanced antioxidant and defense\u2010related responses, as well as rhizosphere soil organic matter and total nitrogen. Together, BS@MCP integrates nanozyme\u2010mediated pathogen suppression with microbial biocontrol, host defense activation and rhizosphere improvement, providing a multifunctional strategy for managing soil\u2010borne crop disease.\n                  <\/jats:p>","DOI":"10.1002\/smll.76287","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T06:38:52Z","timestamp":1792564732000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Multifunctional\n                    <i>Bacillus<\/i>\n                    \u2010Nanozyme Biohybrid for Control of Tobacco Crown Rot Through Cuproptosis\u2010Like Antifungal Activity and Rhizosphere Regulation"],"prefix":"10.1002","author":[{"given":"Anlai","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Sir Run Run Shaw Hospital of Zhejiang University School of Medicine  Hangzhou Zhejiang China"},{"name":"College of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Agro\u2010Food Resources and High\u2010Value Utilization Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5380-0616","authenticated-orcid":false,"given":"Rui","family":"Qiu","sequence":"additional","affiliation":[{"name":"Tobacco Research Institute Key Laboratory for Green Preservation and Control of Tobacco Diseases and Pests in Huanghuai Growing Area Henan Academy of Agricultural Sciences  Xuchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1154-5489","authenticated-orcid":false,"given":"Xiaoxue","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sir Run Run Shaw Hospital of Zhejiang University School of Medicine  Hangzhou Zhejiang China"},{"name":"College of Biosystems Engineering and Food Science Zhejiang Key Laboratory of Agro\u2010Food Resources and High\u2010Value Utilization Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Li","sequence":"additional","affiliation":[{"name":"Tobacco Research Institute Key Laboratory for Green Preservation and Control of Tobacco Diseases and Pests in Huanghuai Growing Area Henan Academy of Agricultural Sciences  Xuchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjun","family":"Li","sequence":"additional","affiliation":[{"name":"Tobacco Research Institute Key Laboratory for Green Preservation and Control of Tobacco Diseases and Pests in Huanghuai Growing Area Henan Academy of Agricultural Sciences  Xuchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sir Run Run Shaw Hospital of Zhejiang University School of Medicine  Hangzhou Zhejiang 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