{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T19:49:03Z","timestamp":1792698543157,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273867"],"award-info":[{"award-number":["82273867"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82504750"],"award-info":[{"award-number":["82504750"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Aggregation\u2010caused quenching (ACQ) probes provide an integrity\u2010reporting strategy for tracking nanocarriers by emitting strong fluorescence when molecularly dispersed within the hydrophobic core of intact carriers and becoming quenched after release into aqueous biological environments. However, previously reported near\u2010infrared\u2010II (NIR\u2010II) ACQ probes suffer from limited effective emission in the NIR\u2010II window and potential fluorescence reillumination after probe release, which may compromise nanoparticle quantification and thereby particokinetic analysis. Here, we developed a series of Flav7\u2010derived cyanine ACQ probes with strong absorption and bright emission in the NIR\u2010II region. By modifying terminal substituents, introducing methoxy groups to modulate intermolecular\n                    <jats:italic>\u03c0\u2013\u03c0<\/jats:italic>\n                    stacking, and screening counterions with different sizes and hydrophobicity, ACQ\u2010F4 was identified as the optimized probe. ACQ\u2010F4 achieved a favorable balance between efficient nanocarrier labeling, water\u2010triggered quenching, and suppressed reillumination in biological environments. Using ACQ\u2010F4 as the probe, the particokinetics and biodistribution of polymeric nanoparticles (PNs) were investigated after intravenous or intraperitoneal administration. Multi\u2010compartment integration of blood, liver, and spleen yielded approximately 90.2% systemic particle exposure after intraperitoneal administration compared with the intravenous reference. In conclusion, this study presents rationally engineered NIR\u2010II ACQ probes for reliable bioimaging and quantification of PNs in vivo, enabling more comprehensive particokinetic assessment beyond blood kinetics alone.\n                  <\/jats:p>","DOI":"10.1002\/smll.76412","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T19:28:36Z","timestamp":1792697316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cyanine\u2010Based Near Infrared\u2010II Aggregation\u2010Caused\u2010Quenching Probes for Particokinetic Analysis"],"prefix":"10.1002","author":[{"given":"Runtong","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, Fudan University Key Laboratory of Smart Drug Delivery of MOE and National Key Laboratory of Advanced Drug Formulations for Overcoming Delivery Barriers  Shanghai People's Republic of 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