{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:00:46Z","timestamp":1790830846782,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Smart Molecules"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Emerging evidence highlights the oncogenic role of the Dishevelled, Egl\u201010, and Pleckstrin domains containing 1 (DEPDC1) gene in lung cancer progression, making it an attractive therapeutic target. In this study, we investigated the anti\u2010cancer potential of Delafloxacin, a fluoroquinolone antibiotic, in DEPDC1\u2010overexpressing non\u2010small cell lung cancer, NCI\u2010H1299 cells. In silico docking of 1399 FDA\u2010approved compounds using AutoDock Vina confirmed the strong binding affinity of Delafloxacin to the DEPDC1 active site (binding energy \u22127.1\u00a0kcal\/mol). Delafloxacin treatment resulted in dose\u2010dependent suppression of cell proliferation, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of approximately 30\u00a0\u03bcg\/mL. RT\u2010qPCR revealed significant downregulation of DEPDC1 and several key regulators of proliferation (RAS, EGFR), mitotic progression (Cyclin\u2010dependent kinase 1 (CDK1), CCNB2, KIF2C), and survival (BIRC5). Cell cycle analysis demonstrated G2\/M phase accumulation upon Delafloxacin treatment, consistent with transcriptional repression of CDK1 and CCNB2. In vivo, Delafloxacin administration (15\u00a0mg\/kg) significantly reduced tumor growth in a xenograft mouse model without overt toxicity. While these findings identify Delafloxacin as a candidate for repurposing in lung cancer, the observed downregulation of DEPDC1 represents a correlative finding rather than validated direct targeting. This study provides a rationale for further investigation of Delafloxacin in DEPDC1\u2010associated malignancies.\n                  <\/jats:p>","DOI":"10.1002\/smo2.70050","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:48:28Z","timestamp":1779961708000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Delafloxacin suppresses DEPDC1 expression and induces G2\/M arrest in non\u2010small cell lung cancer cells: A drug repurposing study"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0000-389X","authenticated-orcid":false,"given":"Noman","family":"Ali","sequence":"first","affiliation":[{"name":"School of Bioengineering Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farishta","family":"Shafiq","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mishal","family":"Iftikhar","sequence":"additional","affiliation":[{"name":"Institute of Advanced Study Shenzhen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Shahzad","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shenzhen University General Hospital Institute of Precision Diagnosis and Treatment of Digestive System Tumors  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2008-9925","authenticated-orcid":false,"given":"Muhammad","family":"Shoaib","sequence":"additional","affiliation":[{"name":"SusMat\u2010RC Mohammed VI Polytechnic University  Ben Guerir Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"59","volume":"2","author":"Singh D. 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