{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T09:59:11Z","timestamp":1780912751750,"version":"3.54.1"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Smart Molecules"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The dysregulation of microRNA (miRNA) expression is closely linked to the pathogenesis of lung cancer, rendering the quantification of trace miRNAs clinically indispensable. However, achieving ultrasensitive detection of miRNAs in complex biological matrices, including living cells, tissues, and blood serum, remains significant challenges. To address this, we developed a fluorogenic detection platform for miR\u201021 based on tetrazine\u2010mediated transfer (TMT) reactions. While TMT strategies have been previously explored, we report a refined probe design optimized through computational screening. By minimizing the energy gap (\u0394\n                    <jats:italic>E<\/jats:italic>\n                    ) between the fluorophore's emissive state and the tetrazine's dark state, we achieved an efficient quenching mechanism with a low background (fluorescence quantum yield &lt;0.01). By integrating hybridization\u2010mediated target recycling with the inverse electron demand Diels\u2010Alder reaction, the Inverse Diels\u2010Alder Cycloaddition Reaction (IDCR) probe achieves signal amplification. Although the theoretical catalytic turnover is significantly enhanced at low target concentrations, the practical detection limit is primarily governed by the high signal\u2010to\u2010background ratio afforded by our optimized probe. This mechanism yields an ultralow limit of detection (3.58\u00a0\u00d7\u00a010\n                    <jats:sup>\u221218<\/jats:sup>\n                    \u00a0M) with a broad linear range (100 aM to 100\u00a0nM). Building upon foundational bioorthogonal chemistry, the IDCR probe enables high\u2010contrast imaging in living cells and tissues and distinguishes lung cancer patients from healthy individuals.\n                  <\/jats:p>","DOI":"10.1002\/smo2.70067","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T09:00:46Z","timestamp":1780909246000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced through\u2010bond energy transfer\u2010based bioorthogonal probe enables catalytic\u2010amplified and sensitive detection of microRNA\u201021 for clinical lung cancer diagnosis"],"prefix":"10.1002","author":[{"given":"Yikun","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuping","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqiao","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering Shenzhen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danhong","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saran","family":"Long","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4316-5350","authenticated-orcid":false,"given":"Wen","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"},{"name":"Ningbo Institute of Dalian University of Technology Dalian University of Technology  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7777-079X","authenticated-orcid":false,"given":"Jianjun","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"},{"name":"Ningbo Institute of Dalian University of Technology Dalian University of Technology  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4962-5186","authenticated-orcid":false,"given":"Jiangli","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Dalian University of Technology  Dalian China"},{"name":"Ningbo Institute of Dalian University of Technology Dalian University of Technology  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ning","sequence":"additional","affiliation":[{"name":"Pancreatic Disease Center Cancer Hospital of Dalian University of Technology Liaoning Cancer Hospital &amp; 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