{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:05:52Z","timestamp":1784181952098,"version":"3.55.0"},"reference-count":74,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Smart Molecules"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Food spoilage driven by microbial growth severely endangers public health and global food security. Traditional methods for food freshness evaluation suffer from cumbersome operation, time\u2010consuming procedures, and poor portability. In contrast, fluorescent probe\u2010based detection offers distinct advantages including high sensitivity, fast response, and visual detectability. This review summarizes recent advances in organic small\u2010molecule fluorescent probes for food freshness assessment over the past 3\u00a0years. We focus on representative sensing mechanisms of these probes, including nucleophilic substitution, protonation\/deprotonation, hydrogen\u2010bonding interactions, Lewis acid\u2010base reactions, and Michael addition as well as typical target analytes such as biogenic amines, hydrogen sulfide, sulfur dioxide derivatives, pH, ATP, and hydrazine. The representative applications of fluorescent probes in portable test strips, smartphone\u2010assisted sensing platforms, and intelligent detection systems are emphatically introduced. Although these probes have achieved remarkable results in sensitivity, response speed and practicality, challenges still exist such as single detection target, insufficient anti\u2010interference ability, and lack of prospective prediction function. Future research will focus on expanding detection scopes, developing multi\u2010target recognition probes, combining artificial intelligence to construct spoilage prediction models, and promoting the industrial application of portable and intelligent food freshness detection technology.<\/jats:p>","DOI":"10.1002\/smo2.70072","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T05:21:28Z","timestamp":1784179288000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Revisiting small\u2010molecule fluorescent probes in food freshness detection"],"prefix":"10.1002","author":[{"given":"Xinrui","family":"Wang","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine College of Health Science and Engineering Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuhan Business University Hubei Provincial Engineering Research Center of Racing Horse Detection and Application Transformation Equine Science Research and Horse Doping Control Laboratory  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Zou","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine College of Health Science and Engineering Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuyuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine College of Health Science and Engineering Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Bian","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine College of Health Science and Engineering Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1766-8886","authenticated-orcid":false,"given":"Zhiqiang","family":"Mao","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine College of Health Science and Engineering Hubei University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1198124","volume":"14","author":"Karanth S.","year":"2023","journal-title":"Front. 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