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Inspired by bio\u2010protection of silk cocoons, a system of silk fibroin (SF) nanococoons is developed, which is capable of providing a bio\u2010protective environment for enzymatic activities on one hand, and facilitating the process of cascade enzymatic reactions on the other hand. This gives rise to the significant enhancement in enzymatic stability and efficacy. For instance, the protected glucose oxidase and horseradish peroxidase enzymes exhibit a 17.5\u2010 and 7.0\u2010fold increase when stored at 60\u2009\u00b0C (&gt;50% activity; \u224828\u2009h) and 25\u2009\u00b0C (&gt;67.5% activity; \u224814\u2009days), respectively, compared with free enzymes. It follows that this protective effect is subject to the mutual templated crystallization between enzymes and SF molecules, which prevents enzyme molecules from unfolding. In animal experiments, it shows that alcohol oxidase and catalase protected by silk nanococoons are able to reduce the alcohol levels of intoxicated mice with &gt;5.0\u2010time efficacy at the alcohol decomposition level of &gt;80% in 4.0\u2009h compared with the unprotected. By bio\u2010inspiration of silk cocoons, the silk bio\u2010nanoreactors demonstrate the significant potential applications in biomedicine.<\/jats:p><\/jats:sec>","DOI":"10.1002\/smsc.202000049","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T03:17:37Z","timestamp":1613013457000},"source":"Crossref","is-referenced-by-count":16,"title":["Silk Nanococoons: Bio\u2010Nanoreactors for Enzymatic Catalytic Reactions and Applications to Alcohol Intoxication"],"prefix":"10.1002","volume":"1","author":[{"given":"Zhengwei","family":"Chen","sequence":"first","affiliation":[{"name":"College of Ocean and Earth Sciences Research Institution for Biomimetics and Soft Matter Fujian Key Provincial Laboratory for Soft Functional Materials Research Xiamen University  422 Siming South Road Xiamen 361005 China"},{"name":"Department of Physics National University of Singapore  2 Science Drive 3 Singapore 117542 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