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Such a structural arrangement is essential to establish 3D stratified epidermis disease models, such as for the autoimmune skin disease pemphigus vulgaris (PV), which targets the cell\u2013cell junctions at the interface of the basal and suprabasal layers. Inspired by epithelial regeneration in wound healing, a method that combines 3D bioprinting and spatially guided self\u2010reorganization of keratinocytes is developed to recapture the fine structural hierarchy that lies in the deep layers of the epidermis. Herein, keratinocyte\u2010laden fibrin hydrogels are bioprinted to create geographical cues, guiding dynamic self\u2010reorganization of cells through collective migration, keratinocyte differentiation, and vertical expansion. This process results in a region of self\u2010organized multilayers (SOMs) that contain the basal\u2010to\u2010suprabasal transition, marked by the expressed levels of different types of keratins that indicate differentiation. 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