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Specifically, their effects in direct co\u2010culture with endothelial cells, pericytes, and myoblasts seeded within microporous 3D scaffolds are characterized. Additionally, the impact of hypoxic PLX\u2010PAD cell\u2010conditioned medium (CM) on vascularization and muscle differentiation of engineered tissue is monitored. Co\u2010culture of PLX\u2010PAD with myocytes stimulated myocyte differentiation while PLX\u2010PAD CM promoted the formation of vascular networks. Implantation of a multi\u2010culture system of vascularized human skeletal muscle tissue and PLX\u2010PAD into a rectus abdominal defect in nude mice promoted myocyte differentiation, host vessel penetration, and tissue integration. 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