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However, it is still a challenge understanding and identifying these molecular mechanisms as biomarkers of disease, because of their high\u2010level of polymorphism and nanoscale dimensions. Here, it provides a review work linking the potential and capabilities of atomic force microscopy (AFM) technologies in unraveling beyond imaging the common and hidden properties of transient and nanosized molecular processes in cancer and neurodegeneration. This study highlights the most prominent operational modes of AFM to achieve morphological, mechanical, and chemical characterization of the molecular processes leading to these diseases. 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