{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:38:13Z","timestamp":1790876293126,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Small Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:p>\n                    The ability to sensitively and specifically detect cancer\u2010associated nucleic acids carrying single\u2010nucleotide mutations is critical for early cancer detection, patient stratification, and personalized treatment, particularly through non\u2010invasive liquid biopsy approaches. Detecting low\u2010abundance nucleic acid fragments\u2014particularly those with single\u2010nucleotide variations\u2014remains a significant challenge for point\u2010of\u2010care (POC) diagnostics. Here, we report a programmable DNA\u2010based self\u2010assembly strategy that leverages primer exchange reaction (PER) for isothermal signal amplification and enables colorimetric detection of cancer\u2010specific DNA and RNA fragments on gold nanoparticle\u2010based lateral flow assays (LFAs). This method uses PER\u2010generated DNA concatemers functionalized with multiple FITC\u2010labeled imager strands to enhance the visual signal on conventional LFA strips. We demonstrate that this approach enables detection of a synthetic\n                    <jats:italic>P53<\/jats:italic>\n                    oncogene fragment with a limit of detection as low as 16\u2009pM, representing a 16\u2010fold improvement over single\u2010dye labeled controls. The system also reliably distinguishes single\u2010nucleotide mutations at 10% relative abundance within a wild\u2010type background. Moreover, we show successful detection of mutant fragments in complex biological fluids such as serum and saliva, as well as of RNA extracted from breast cancer cell lines and RNA derived from circulating tumor DNA (ctDNA) from patient plasma samples. Specifically, we detect clinically relevant\n                    <jats:italic>PIK3CA<\/jats:italic>\n                    E545K\/A and\n                    <jats:italic>P53<\/jats:italic>\n                    R280K mutations, consistent with Sanger sequencing results and validating our method for liquid biopsy applications. Overall, this PER\u2010based self\u2010assembly system provides a simple, robust, and sensitive platform for mutation\u2010specific nucleic acid detection using LFAs and offers strong potential for translation into laboratory research applications and POC diagnostics workflows for cancer and other genetic disorders.\n                  <\/jats:p>","DOI":"10.1002\/smsc.202500520","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T20:39:03Z","timestamp":1770410343000},"source":"Crossref","is-referenced-by-count":1,"title":["Detection of Cancer\u2010Associated Mutations Using Primer Exchange Reaction\u2010Based Signal Amplification and Lateral Flow Assays"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3159-4855","authenticated-orcid":false,"given":"Samet","family":"Kocabey","sequence":"first","affiliation":[{"name":"Laboratory of Experimental and Translational Oncology Department of Oncology Microbiology and Immunology Faculty of Science and Medicine University of Fribourg  Fribourg Switzerland"},{"name":"NCCR Bio\u2010inspired Materials University of Fribourg  Fribourg Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9137-7695","authenticated-orcid":false,"given":"Curzio","family":"R\u00fcegg","sequence":"additional","affiliation":[{"name":"NCCR Bio\u2010inspired Materials University of Fribourg  Fribourg Switzerland"},{"name":"Xemperia SA, Marly Innovation Centre  Marly 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