{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:02:31Z","timestamp":1789491751681,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["444711651"],"award-info":[{"award-number":["444711651"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RTG 2723 Materials\u2013Microbes\u2013Microenvironments (M\u2010M\u2010M)"],"award-info":[{"award-number":["RTG 2723 Materials\u2013Microbes\u2013Microenvironments (M\u2010M\u2010M)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Small Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Biomaterial\u2010associated infections (BAIs) and insufficient early cellular response remain critical challenges for orthopedic implants. We introduce a comprehensive study that bridges current knowledge gaps by examining an early\u2010stage antimicrobial effect on the clinically relevant alloy Ti\u20136Al\u20134V. It combines pathogenic strains with parallel osteoblast assays and tilted\u2010view SEM analysis to obtain a qualitative understanding of the adhesion mechanisms. Detailed physicochemical characterization revealed a progressive increase in nanoscale roughness and oxide layer thickness, accompanied by selective Al\/V depletion and pronounced hydrophilization. To evaluate biological responses, we used standardized in vitro models with\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    , and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . Bacterial adhesion was quantified by SYTO9 staining, a GFP\u2010expressing strain as a viability control, and SEM imaging. Nanostructured (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>q<\/jats:sub>\n                    \u2009\u2264\u200940\u2009nm) surfaces significantly reduced early bacterial attachment compared to polished nanoflat controls. In parallel, osteoblast\u2010like SaOs\u20102 cells showed stable adhesion and spreading, confirmed by phalloidin\/DAPI staining and LDH cytotoxicity assay. Together, these results demonstrate that NaOH\u2010etched Ti\u20136Al\u20134V surfaces can impair early microbial adhesion based on physical action and preserve osteoblast compatibility. By integrating advanced materials characterization with microbiological and cell biological assays, we provide a framework for topography\u2010driven surface design toward infection\u2010resistant orthopedic implants that support favorable early osteoblast\u2013surface interactions.\n                  <\/jats:p>","DOI":"10.1002\/smsc.70348","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:50:09Z","timestamp":1785300609000},"source":"Crossref","is-referenced-by-count":0,"title":["Nanostructured Ti\u20136Al\u20134V Reduces Adhesion of Several Bacterial Species: An In Vitro Study"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9127-4758","authenticated-orcid":false,"given":"Sadaf","family":"Khalatbarizamanpoor","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology Jena University Hospital  Jena Germany"},{"name":"Excellence Graduate School \u201cJena School f\u00fcr Microbial Communication (JSMC)\u201d Friedrich Schiller University Jena  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8110-6764","authenticated-orcid":false,"given":"Adrian G.","family":"Nowotnick","sequence":"additional","affiliation":[{"name":"Excellence Graduate School \u201cJena School f\u00fcr Microbial Communication (JSMC)\u201d Friedrich Schiller University Jena  Jena Germany"},{"name":"Otto Schott Institute of Materials Research Friedrich Schiller University Jena  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8250-4696","authenticated-orcid":false,"given":"Stephanie","family":"Lippmann","sequence":"additional","affiliation":[{"name":"Institute of Applied Physics Friedrich Schiller University Jena  Jena 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