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The amphiphiles combine asymmetrically substituted azobenzene cores with ionizable and lipophilic dendrons and undergo efficient, reversible\n                    <jats:italic>E<\/jats:italic>\n                    \/\n                    <jats:italic>Z<\/jats:italic>\n                    photoisomerization under biologically relevant conditions. In the presence of pDNA, they form nanocomplexes displaying markedly different supramolecular organizations depending on the architecture of the ionizable domain, ranging from highly ordered lamellar nanoparticles to morula\u2010like or weakly structured condensates. Molecular mechanics and molecular dynamics simulations reveal that photoisomerization within preassembled lamellar dendriplexes introduces local structural frustration, transiently destabilizes bilayer organization, and enhances amphiphile dissociation. These structural perturbations correlate with enhanced intracellular delivery and transgene expression upon\n                    <jats:italic>E\u2009<\/jats:italic>\n                    \u2194\u2009\n                    <jats:italic>Z<\/jats:italic>\n                    interconversion. In vitro,\n                    <jats:italic>Z<\/jats:italic>\n                    \u2010derived nanocomplexes systematically outperform the corresponding\n                    <jats:italic>E<\/jats:italic>\n                    \u2010isomers in COS\u20107, HepG2, and RAW264.7 cells, while ex vivo photoswitching yields up to 50\u2010fold increases in expression. In vivo studies further demonstrate isomer\u2010dependent passive organ targeting, including pronounced shifts from lung\u2010 to liver\u2010dominant transfection profiles. Collectively, these results establish molecular photoswitching as a mechanism to encode supramolecular DNA organization and biological function in single\u2010component dendritic vectors.\n                  <\/jats:p>","DOI":"10.1002\/smsc.70377","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T06:29:12Z","timestamp":1786602552000},"source":"Crossref","is-referenced-by-count":1,"title":["Azobenzene\u2010Cored Amphiphilic Dendrimers: Molecular Photoswitching Controls Dendriplex Topology and Gene Delivery"],"prefix":"10.1002","volume":"6","author":[{"given":"Mar\u00eda J.","family":"Puerto\u2010Madorr\u00e1n","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmicas (IIQ) CSIC \u2013 Universidad de Sevilla  Seville Spain"},{"name":"Photophysique et Photochimie Supramol\u00e9culaires et Macromol\u00e9culaires Universit\u00e9 Paris\u2010Saclay, ENS Paris\u2010Saclay, CNRS  Gif\u2010sur\u2010Yvette 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