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However, the optical throughput of the microscope limits the available information per unit of time, i.e., it is difficult to obtain both high spatial and temporal resolution at once. Here, a method is proposed to construct a kind of intravital microscopy with high optical throughput, by making near\u2010infrared\u2010II (NIR\u2010II, 900\u20131880\u00a0nm) wide\u2010field fluorescence microscopy learn from two\u2010photon fluorescence microscopy based on a scale\u2010recurrent network. Using this upgraded NIR\u2010II fluorescence microscope, vessels in the opaque brain of a rodent are reconstructed three\u2010dimensionally. Five\u2010fold axial and thirteen\u2010fold lateral resolution improvements are achieved without sacrificing temporal resolution and light utilization. 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