{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:57:59Z","timestamp":1789181879886,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171296\u2010T.C."],"award-info":[{"award-number":["32171296\u2010T.C."]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["82225025\u2010T.C."],"award-info":[{"award-number":["82225025\u2010T.C."]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Fundamental Research Program","doi-asserted-by":"publisher","award":["JCYJ20200109142019319"],"award-info":[{"award-number":["JCYJ20200109142019319"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inactivated virus vaccines with whole antigen spectra and good safety are the commonly used modality for preventing infections. However, the poor immunogenicity greatly limits its clinical applications. Herein, by taking advantages of the crucial roles of Se in the functions of immune cells and its biomineralization property, it successfully in\u2010situ synthesized Se nanoadjuvant on inactivated viruses such as porcine epidemic diarrhea virus (PEDV), pseudorabies virus (PRV), and porcine reproductive and respiratory syndrome virus (PRRSV) in a facile method, which is universal to construct other inactivated virus vaccines. The nanovaccine can highly effectively enhance the uptake of PEDV\/PRV\/PRRSV into dendritic cells (DCs) and activate DCs via triggering TLR4 signaling pathways and regulating selenoproteins expressions. Furthermore, it exhibited better activities in triggering macrophages and natural killer cells\u2010mediated innate immunity and T cells\u2010mediated cellular immunity compared to PEDV and the commercial inactivated PEDV vaccine on both mice and swine models. This study provides a universal Se nanoadjuvant for developing inactivated viruses\u2010based nanovaccines for preventing virus infections.<\/jats:p>","DOI":"10.1002\/smtd.202300293","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T05:00:05Z","timestamp":1690347605000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Designing Selenium Nanoadjuvant as Universal Agent for Live\u2010Killed Virus\u2010Based Vaccine"],"prefix":"10.1002","volume":"7","author":[{"given":"Bo","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Chemistry Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan University  Guangzhou Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"He","sequence":"additional","affiliation":[{"name":"Wens Foodstuff Group Co. Ltd  Yunfu Guangdong 527400 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongdong","family":"Wang","sequence":"additional","affiliation":[{"name":"Wens Foodstuff Group Co. Ltd  Yunfu Guangdong 527400 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan University  Guangzhou Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Chemistry Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan University  Guangzhou Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6953-1342","authenticated-orcid":false,"given":"Tianfeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan University  Guangzhou Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4946-2567","authenticated-orcid":false,"given":"Ligeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan University  Guangzhou Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32588-3"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2020.198045"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06056-w"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2023.109666"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2019.00347"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-019-04167-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.0c01252"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2022.09.013"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxu063"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-020-0737-y"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-022-01098-0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201300583"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b09041"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202110905"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202111670"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202205193"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202201409"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202201595"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl300027p"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NR09208F"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.931232"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-00478-8"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00996-0"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.07.004"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101715"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.622898"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119377"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119397"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10091203"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2015.03.004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c05558"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-021-00905-5"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b00978"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-022-01296-w"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c09033"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI163449"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202105742"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202201087"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202101391"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202201518"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01371-1"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-023-01325-x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abl5278"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202200289"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2021.100290"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2015.9"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0034-2"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj1249"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01809-14"}],"container-title":["Small Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.202300293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T20:22:29Z","timestamp":1700079749000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smtd.202300293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,25]]},"references-count":49,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/smtd.202300293"],"URL":"https:\/\/doi.org\/10.1002\/smtd.202300293","archive":["Portico"],"relation":{},"ISSN":["2366-9608","2366-9608"],"issn-type":[{"value":"2366-9608","type":"print"},{"value":"2366-9608","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,25]]},"assertion":[{"value":"2023-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300293"}}