{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:55:56Z","timestamp":1791449756665,"version":"4.3.3"},"reference-count":85,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T00:00:00Z","timestamp":1699142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T00:00:00Z","timestamp":1699142400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100017400","name":"Finnish Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017400","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010711","name":"Sy\u00f6p\u00e4s\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003125","name":"Suomen Kulttuurirahasto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007083","name":"Orionin Tutkimuss\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007083","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9liuksen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004012","name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004012","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cells are highly dynamic and adopt variable shapes and sizes. These variations are biologically important but challenging to investigate in a spatiotemporally controlled manner. Micropatterning, confining cells on microfabricated substrates with defined geometries and molecular compositions, is a powerful tool for controlling cell shape and interactions. However, conventional binary micropatterns are static and fail to address dynamic changes in cell polarity, spreading, and migration. Here, a method for dynamic micropatterning is reported, where the non\u2010adhesive surface surrounding adhesive micropatterns is rapidly converted to support specific cell\u2010matrix interactions while allowing simultaneous imaging of the cells. The technique is based on ultraviolet photopatterning of biotinylated polyethylene glycol\u2010grafted poly\u2010L\u2010lysine, and it is simple, inexpensive, and compatible with a wide range of streptavidin\u2010conjugated ligands. Experiments using biotinylation\u2010based dynamic micropatterns reveal that distinct extracellular matrix ligands and bivalent integrin\u2010clustering antibodies support different degrees of front\u2010rear polarity in human glioblastoma cells, which correlates to altered directionality and persistence upon release and migration on fibronectin. Unexpectedly, however, neither an asymmetric cell shape nor centrosome orientation can fully predict the future direction of migration. Taken together, biotinylation\u2010based dynamic micropatterns allow easily accessible and highly customizable control over cell morphology and motility.<\/jats:p>","DOI":"10.1002\/smtd.202300719","type":"journal-article","created":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T22:56:05Z","timestamp":1699224965000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Dynamic Micropatterning Reveals Substrate\u2010Dependent Differences in the Geometric Control of Cell Polarization and Migration"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6006-9163","authenticated-orcid":false,"given":"Aleksi","family":"Isomursu","sequence":"first","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7698-3061","authenticated-orcid":false,"given":"Jonna","family":"Alanko","sequence":"additional","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0227-1045","authenticated-orcid":false,"given":"Sara","family":"Hern\u00e1ndez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"},{"name":"Institute of Biomedicine and MediCity Research Laboratories University of Turku  Turku 20014 Finland"},{"name":"Department of Life Technologies University of Turku  Turku 20520 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla","family":"Saukkonen","sequence":"additional","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markku","family":"Saari","sequence":"additional","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2805-0686","authenticated-orcid":false,"given":"Pieta K.","family":"Mattila","sequence":"additional","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"},{"name":"Institute of Biomedicine and MediCity Research Laboratories University of Turku  Turku 20014 Finland"},{"name":"Department of Life Technologies University of Turku  Turku 20520 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6295-6556","authenticated-orcid":false,"given":"Johanna","family":"Ivaska","sequence":"additional","affiliation":[{"name":"Turku Bioscience Centre University of Turku and \u00c5bo Akademi University  Turku 20520 Finland"},{"name":"Department of Life Technologies University of Turku  Turku 20520 Finland"},{"name":"InFLAMES Research Flagship Center University of Turku  Turku 20520 Finland"},{"name":"Western Finnish Cancer Center (FICAN West) University of Turku  Turku 20520 Finland"},{"name":"Foundation for the Finnish Cancer Institute  Helsinki 00014 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,5]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609267103"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110494"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336918.2016.1148864"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5317.1425"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.12.004"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0237-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.075150"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm0493382"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.04.059"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.12.012"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.104901"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201300902"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja038265b"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja029485c"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408954102"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/la102955m"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2000.41.6.803"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.06.005"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9532"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.738786"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201504154"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/la404037s"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200801864"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.01.029"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.03.032"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201204474"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nphys1306"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/la2000206"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02827"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120548"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.347"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201612069"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.022"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201003091"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.06.004"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.2.589"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.8.2603"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.33864"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69229"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015462"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2975"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.225805"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-021-01108-x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69229"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2019.10.004"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.02.034"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e17-08-0488"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.01.038"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00471-8"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.2.265"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90056-6"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-05-0435"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3026"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/b821581m"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)84259-J"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.05.008"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-022-01294-2"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12257"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100157"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201694177"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.72805"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.7b02935"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.08.020"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2005.12.001"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2019.10.001"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7846531"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202201388"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201900041"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(91)82027-6"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1163595"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3191"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.01.056"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9674"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20230211"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf7119"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4352"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.01.027"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1021\/la026394k"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.2.474"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-018-0216-7"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22518-0"},{"key":"e_1_2_9_83_1","unstructured":"U.Lund C.Agostinelli H.Arai A.Gagliardi E.Garc\u00eda\u2010Portugu\u00e9s D.Giunchi J.\u2010O.Irisson M.Pocernich F.Rotolo https:\/\/CRAN.R\u2010project.org\/package=circular(accessed: December2022)."},{"key":"e_1_2_9_84_1","unstructured":"S.Chakraborty S. W. K.Wong https:\/\/CRAN.R\u2010project.org\/package=BAMBI(accessed: December2022)."},{"key":"e_1_2_9_85_1","unstructured":"H.Wickham W.Chang L.Henry T. L.Pedersen K.Takahashi C.Wilke K.Woo H.Yutani D.Dunnington https:\/\/CRAN.R\u2010project.org\/package=ggplot2(accessed: December2022)."}],"container-title":["Small Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.202300719","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smtd.202300719","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.202300719","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:38:54Z","timestamp":1791448734000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smtd.202300719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,5]]},"references-count":85,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/smtd.202300719"],"URL":"https:\/\/doi.org\/10.1002\/smtd.202300719","archive":["Portico"],"relation":{},"ISSN":["2366-9608","2366-9608"],"issn-type":[{"value":"2366-9608","type":"print"},{"value":"2366-9608","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,5]]},"assertion":[{"value":"2023-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300719"}}