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However, the sample size is limited to only a few hundred cells. Here, a droplet\u2010based scGR\u2010seq is developed to address this issue by adopting a 10x Chromium platform to simultaneously profile ten thousand cells' glycome and transcriptome in single cells. To establish droplet\u2010based scGR\u2010seq, a comparative analysis of two distinct cell lines is performed: pancreatic ductal adenocarcinoma cells and normal pancreatic duct cells. Droplet\u2010based scGR\u2010seq revealed distinct glycan profiles between the two cell lines that showed a strong correlation with the results obtained by flow cytometry. Next, droplet\u2010based scGR\u2010seq is applied to a more complex sample: peripheral blood mononuclear cells (PBMC) containing various immune cells. The method can systematically map the glycan signature for each immune cell in PBMC as well as glycan alterations by cell lineage. Prediction of the association between the glycan expression and the gene expression using regression analysis ultimately leads to the identification of a glycan epitope that impacts cellular functions. In conclusion, the droplet\u2010based scGR\u2010seq realizes the high\u2010throughput profiling of the distinct cellular glyco\u2010states in single cells.<\/jats:p>","DOI":"10.1002\/smtd.202301338","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T15:59:21Z","timestamp":1704211161000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Droplet\u2010Based Glycan and RNA Sequencing for Profiling the Distinct Cellular Glyco\u2010States in Single Cells"],"prefix":"10.1002","volume":"8","author":[{"given":"Sunanda","family":"Keisham","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute, Multicellular System Regulation Research Group National Institute of Advanced Industrial Science and Technology (AIST)  Central 6, 1\u20101\u20101 Higashi Tsukuba Ibaraki 305\u20138566 Japan"},{"name":"Ph.D. Program in Human Biology, School of Integrative and Global Majors University of Tsukuba  Tsukuba Ibaraki 305\u20108566 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayoko","family":"Saito","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute, Multicellular System Regulation Research Group National Institute of Advanced Industrial Science and Technology (AIST)  Central 6, 1\u20101\u20101 Higashi Tsukuba Ibaraki 305\u20138566 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satori","family":"Kowashi","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute, Multicellular System Regulation Research Group National Institute of Advanced Industrial Science and Technology (AIST)  Central 6, 1\u20101\u20101 Higashi Tsukuba Ibaraki 305\u20138566 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3006-1659","authenticated-orcid":false,"given":"Hiroaki","family":"Tateno","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biotechnology Research Institute, Multicellular System Regulation Research Group National Institute of Advanced Industrial Science and Technology (AIST)  Central 6, 1\u20101\u20101 Higashi Tsukuba Ibaraki 305\u20138566 Japan"},{"name":"Ph.D. Program in Human Biology, School of Integrative and Global Majors University of Tsukuba  Tsukuba Ibaraki 305\u20108566 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,2]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cww086"},{"key":"e_1_2_9_2_1","unstructured":"A.Varki R. 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