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However, methods to enable diagnostics of non\u2010nucleic\u2010acid targets with these systems are still limited. Herein, by fusing ligand\u2010dependent allosteric ribozymes with CRISPR\u2010Cas12a, a derived CRISPR\u2010Cas system is created for efficient quantitative analysis of non\u2010nucleic\u2010acid targets in 1\u20132\u00a0h. On two different small molecules, the system's generality, reliability and accuracy is demonstrated, and show that the well operability of this system can enable high\u2010throughput detection of a small molecule in blood samples. The system can be further converted to rely on allosteric deoxyribozyme instead of allosteric ribozyme to recognize non\u2010nucleic\u2010acid targets and transduce the signal to CRISPR\u2010Cas12a for amplification, likely making it easier for storage and more consistent in data generation as DNA possess a stability advantage over RNA. This (deoxy)ribozyme\u2010assisted CRISPR\u2010Cas12a system anticipates that it can facilitate bioanalysis in various scientific and clinical settings and further drive the development of clinical translation.<\/jats:p>","DOI":"10.1002\/smtd.202401236","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T03:54:46Z","timestamp":1729223686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Fusing Allosteric Ribozymes with CRISPR\u2010Cas12a for Efficient Diagnostics of Small Molecule Targets"],"prefix":"10.1002","volume":"9","author":[{"given":"Lichuan","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Breast Cancer in Shanghai Department of Breast Surgery and Department of gynecologic oncology Institutes of Biomedical Sciences Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"},{"name":"Department of Chemical Biology School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Breast Cancer in Shanghai Department of Breast Surgery and Department of gynecologic oncology Institutes of Biomedical Sciences Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Breast Cancer in Shanghai Department of Breast Surgery and Department of gynecologic oncology Institutes of Biomedical Sciences Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"},{"name":"State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers National Clinical Research Center for Digestive Diseases Xijing Hospital Fourth Military Medical University  Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Breast Cancer in Shanghai Department of Breast Surgery and Department of gynecologic oncology Institutes of Biomedical Sciences Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5058-4815","authenticated-orcid":false,"given":"Hongzhou","family":"Gu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Breast Cancer in Shanghai Department of Breast Surgery and Department of gynecologic oncology Institutes of Biomedical Sciences Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University  Shanghai 200032 China"},{"name":"Department of Chemical Biology School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine Shanghai Jiao Tong University  Shanghai 200240 China"},{"name":"Carbon\u2010Negative Synthetic Biology for Biomaterial Production from CO2 (CNSB) Campus for Research Excellence and Technological Enterprise (CREATE)  1 CREATE Way Singapore 138602 Singapore"},{"name":"Xiangfu Laboratory  Jiashan 314102 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