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Despite advancements in novel antimicrobial agents, many fail to comprehensively cover common resistant bacterial strains or undergo rigorous multi\u2010center validation. Herein, a cationic AIE\u2010active photosensitizers are developed, ITPM, derived from a triphenylamine\u2010pyridine backbone to address the MDR challenge. Rigorous validation demonstrates that ITPM possesses superior fluorescence imaging capabilities and exceptional antibacterial efficacy. And its broad\u2010spectrum activity is verified through a multi\u2010center study involving six clinically relevant MDR strains. Additionally, resistance development studies and comparisons with advanced clinical antibiotics reveal that ITPM exhibits potent, broad\u2010spectrum antimicrobial activity with minimal resistance development. This efficacy is attributed to its unique antibacterial mechanism involving disrupting bacterial internal structures. These findings establish ITPM as a promising candidate for broad\u2010spectrum antimicrobial therapy, offering a potential solution to the growing crisis of AMR in clinical settings.<\/jats:p>","DOI":"10.1002\/smtd.202401663","type":"journal-article","created":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T01:53:01Z","timestamp":1736560381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["AIE\u2010Active Antibacterial Photosensitizer Disrupting Bacterial Structure: Multicenter Validation against Drug\u2010Resistant Pathogens"],"prefix":"10.1002","volume":"9","author":[{"given":"Guanqing","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Guangdong Provincial Key Laboratory of Precision Medical Diagnostics Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors Guangdong Provincial Key Laboratory of Single Cell Technology and Application Nanfang Hospital Southern Medical University  Guangzhou 510515 P. 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