{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:19:33Z","timestamp":1791397173584,"version":"4.3.3"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,2,2]],"date-time":"2025-02-02T00:00:00Z","timestamp":1738454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["2021SHZDZX"],"award-info":[{"award-number":["2021SHZDZX"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["20DZ2220400"],"award-info":[{"award-number":["20DZ2220400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22204103"],"award-info":[{"award-number":["22204103"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>With the rising incidence of benign prostatic hyperplasia (BPH) due to societal aging, accurate and early diagnosis has become increasingly critical. The clinical challenges associated with BPH diagnosis, particularly the lack of specific biomarkers that can differentiate BPH from other causes of lower urinary tract symptoms (LUTS). Here, matrix\u2010assisted laser desorption\/ionization mass spectrometry (MALDI MS) metabolomic detection platform utilizing urine and serum samples is applied to explore metabolic information and identify potential biomarkers in designed cohort. The nanoparticle\u2010assisted platform demonstrated rapid analysis, minimal sample consumption, and high reproducibility. Employing a two\u2010step grouping screening approach, the identification of urinary metabolic patterns (UMPs) is automated to distinguish healthy individuals from LUTS group, followed by the use of serum metabolic patterns (SMPs) to accurately identify BPH cases within the LUTS cohort, achieving an area under the curve (AUC) of 0.830 (95% CI: 0.802\u20100.851). Furthermore, eight BPH\u2010sensitive metabolic markers are identified, confirming their uniform distribution across age groups (<jats:italic>p<\/jats:italic> &gt; 0.05). This research contributes valuable insights for the early diagnosis and personalized treatment of BPH, enhancing clinical practice and patient care.<\/jats:p>","DOI":"10.1002\/smtd.202401906","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T02:17:46Z","timestamp":1738549066000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Decoding Benign Prostatic Hyperplasia: Insights from Multi\u2010Fluid Metabolomic Analysis"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6744-4822","authenticated-orcid":false,"given":"Xiaoyu","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. China"},{"name":"State Key Laboratory for Oncogenes and Related Genes School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University  Shanghai 200030 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haisong","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. China"},{"name":"Department of Assisted Reproduction Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanshan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Oncogenes and Related Genes School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University  Shanghai 200030 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Oncogenes and Related Genes School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University  Shanghai 200030 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxi","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Oncogenes and Related Genes School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University  Shanghai 200030 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuning","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Oncogenes and Related Genes School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University  Shanghai 200030 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1666-1965","authenticated-orcid":false,"given":"Kun","family":"Qian","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Oncogenes and Related Genes School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University  Shanghai 200030 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 200011 P. R. 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