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Herein, the integrated portable and automatic digital detection system (IPADS), a novel diagnostic platform for nucleic acid detection is introduced. The device employs the hybrid magnetic system (HMS), which uses an electromagnet and a movable permanent magnet to modulate the magnetic field and control bead movement, increasing nucleic acid extraction efficiency to over 80%, while simplifying the traditional labor\u2010intensive process and enabling quick, low\u2010risk sample processing. Additionally, a disposable cartridge is designed for integrated HMS based preprocessing, with detection performed using digital RPA\u2010Cas12a, enabling rapid, enclosed, and automation\u2010friendly detection across a dynamic range spanning five orders of magnitude, with a sensitivity as low as 100 copies mL<jats:sup>\u22121<\/jats:sup> in serum samples. An automated platform further optimizes workflow. As a proof of concept, IPADS is applied to detect hepatitis B virus (HBV) DNA in 20 clinical serum samples, demonstrating high concordance with gold\u2010standard quantitative PCR (qPCR) methods. These results validate the potential of IPADS as a reliable point\u2010of\u2010care testing solution.<\/jats:p>","DOI":"10.1002\/smtd.202401971","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T07:15:47Z","timestamp":1735802147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Integrated, Portable, and Automatic Digital Detection System for Hepatitis B Virus Using Hybrid Magnetic System"],"prefix":"10.1002","volume":"9","author":[{"given":"Xiaoying","family":"Han","sequence":"first","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"},{"name":"School of Life Sciences Zhejiang University  Hangzhou Zhejiang Province 310058 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juxin","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"},{"name":"School of Information and Electrical Engineering Hangzhou City University  Hangzhou Zhejiang Province 310015 China"},{"name":"Academy of Edge Intelligence Hangzhou City University  Hangzhou Zhejiang Province 310015 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianjian","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province Affiliated Hangzhou First People's Hospital School of Medicine Westlake University  Hangzhou Zhejiang Province 310006 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yehong","family":"Gui","sequence":"additional","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"},{"name":"School of Life Sciences Zhejiang University  Hangzhou Zhejiang Province 310058 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haohua","family":"Mei","sequence":"additional","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"},{"name":"School of Life Sciences Zhejiang University  Hangzhou Zhejiang Province 310058 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jizhi","family":"Tong","sequence":"additional","affiliation":[{"name":"School of Information and Electrical Engineering Hangzhou City University  Hangzhou Zhejiang Province 310015 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4322-2522","authenticated-orcid":false,"given":"Ying","family":"Mu","sequence":"additional","affiliation":[{"name":"College of Control Science and Engineering State Key Laboratory of Industrial Control Technology Zhejiang University  Hangzhou Zhejiang Province 310027 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,2]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(03)00023-8"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2023.108141"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00598-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41378-024-00677-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2022.123711"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-023-01092-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02401-6"},{"key":"e_1_2_8_6_2","first-page":"160","volume":"16","author":"Parkin N. 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