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Liquid biopsy, valued for its minimal invasiveness, is widely utilized in cancer detection. However, traditional methods necessitate protein and lipid removal while suffering from low throughput and sensitivity. To address these limitations, a novel magnetic chemoselective probe, Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    \u2010Polymers\u2010Cystamine\u2010Boclys\u2010Gly\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    \u2010PITC (FPBG\u2010PITC), is synthesized, incorporating hydrophobic tags to improve metabolite detection sensitivity and chromatographic separation. Using this probe, a high\u2010throughput metabolic analysis platform is established, allowing efficient enrichment, purification, and quantification of serum amino\u2010containing metabolites with reduced costs. Through comparative analysis of serum samples from healthy controls and patients with benign or malignant breast lesions, four metabolites\u2014asparagine, tryptophan, leucine\/isoleucine, and homocitrulline\u2014are identified as potential biomarkers for early breast disease screening, providing a novel diagnostic strategy.\n                  <\/jats:p>","DOI":"10.1002\/smtd.202500297","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T07:25:22Z","timestamp":1753773922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A High\u2010Throughput Platform Utilizing FPBG\u2010PITC for Amino\u2010Containing Metabolites Screening in Breast Diseases"],"prefix":"10.1002","volume":"9","author":[{"given":"Hongtao","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Biophysics and Structural Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing 100005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Institute of Basic Medical Sciences Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing 100005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunhua","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College  Beijing 100005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Chunhua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College  Beijing 100005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2982-2601","authenticated-orcid":false,"given":"Jiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Analytical Instrumentation Center College of Chemistry and Molecular Engineering Peking University  Beijing 100871 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1190-7949","authenticated-orcid":false,"given":"Zhili","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Structural Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing 100005 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-019-02705-6"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-0375-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.603837"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2022.100500"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA08985C"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2015.08.015"},{"journal-title":"J. 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