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A critical challenge is preventing unwanted molecular transfer (cross\u2010pollination) between different probe patterns, which compromises measurement accuracy. This study investigates cross\u2010pollination in multiplexed biosensing arrays fabricated using thermal scanning probe lithography (tSPL), a method that offers sub\u201010\u00a0nm spatial resolution. By systematically optimizing both the tSPL patterning parameters and chemical functionalization protocols, particularly biomolecule incubation times, this work demonstrates the ability to reduce cross\u2010pollination to undetectable levels (0%). These experimental results establish a clear relationship between processing conditions and cross\u2010contamination rates, providing a practical framework for reliable nanoscale patterning of multiple biomolecular probes on a single surface. These findings enable the development of high\u2010fidelity, multi\u2010target biosensing platforms with enhanced specificity, and therefore sensitivity, for applications in medical diagnostics, environmental monitoring, and biological research.<\/jats:p>","DOI":"10.1002\/smtd.202500963","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T05:34:36Z","timestamp":1761284076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Controlling Cross\u2010Pollination of Biosensing Probes in Nanoscale Multiplexed Arrays"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5907-2816","authenticated-orcid":false,"given":"Hashem Hassan","family":"Nasralla","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Tandon School of Engineering New York University  Brooklyn NY 11201 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