{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T17:34:28Z","timestamp":1793208868509,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,21]],"date-time":"2025-12-21T00:00:00Z","timestamp":1766275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,21]],"date-time":"2025-12-21T00:00:00Z","timestamp":1766275200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1200900"],"award-info":[{"award-number":["2021YFA1200900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA1207300"],"award-info":[{"award-number":["2022YFA1207300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["B2023203026"],"award-info":[{"award-number":["B2023203026"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Peroxynitrite (ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) is a key mediator of redox imbalance in inflammation and cancer, with in vivo monitoring of its dynamic behavior still posing a major challenge. Here, we developed a BD@PEG nanoprobe that enabled dual\u2010excitation NIR\u2010II ratiometric imaging with superior biocompatibility, quantitative fidelity, and ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    selectivity. When applied to a drug\u2010induced liver injury model, BD@PEG\u2010based NIR\u2010II ratiometric imaging allowed real\u2010time monitoring of hepatic ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    dynamics, directly correlating oxidative\/nitrosative stress to histopathological alterations, and demonstrating significant attenuation under glutathione treatment. In a CT26 tumor model, the NIR\u2010II ratiometric imaging strategy revealed distinct ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    modulation in response to different therapeutic interventions. Furthermore, two\u2010plex imaging by integrating NIR\u2010IIb oxyhemoglobin saturation analysis afforded concurrent assessment of nitrosative stress and vascular oxygenation. We found that the combination of chemotherapy and immunotherapy induced synergistic ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    production, enhanced immune cell infiltration, and achieved superior antitumor efficacy. Overall, this study established a versatile imaging platform for dynamic redox monitoring and oxygenation assessment in vivo, providing mechanistic insight into ONOO\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2010mediated pathology and a translational tool for optimizing therapeutic interventions in cancer and inflammation.\n                  <\/jats:p>","DOI":"10.1002\/smtd.202501888","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T06:06:07Z","timestamp":1766383567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo NIR\u2010II Ratiometric Imaging of Peroxynitrite for Tracking Redox Dynamics in Tumor Immunochemotherapy"],"prefix":"10.1002","volume":"10","author":[{"given":"Qikang","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Metastable Materials Science and Technology (MMST) Hebei Key Laboratory of Applied Chemistry Yanshan University  Qinhuangdao China"},{"name":"CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Eldoksh","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"Department of Biotechnology Institute of Graduate Studies and Research Alexandria University  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfan","family":"Lu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Xie","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingrun","family":"Yang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences  Beijing China"},{"name":"The First Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Gu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Metastable Materials Science and Technology (MMST) Hebei Key Laboratory of Applied Chemistry Yanshan University  Qinhuangdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3712-2063","authenticated-orcid":false,"given":"Yeteng","family":"Zhong","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423\u2010020\u201000556\u2010w"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.06.025"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201012\u20101554"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00568"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2222"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.519"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.09.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010021\u201001486\u20105"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202301455"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.113904"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c17899"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.80529"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202206894"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6SC00012F"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201604764"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201029894\u20101"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566\u2010024\u201001391\u20105"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010024\u201000892\u20100"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202007040"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202503885"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.3c00495"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202009942"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202400132"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c10659"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b08457"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202418378"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b00317"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806153115"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/14686996.2019.1590731"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566\u2010025\u201001736\u20108"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00612H"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010023\u201001501\u20104"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr078381n"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141122274"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0TB01970D"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.3c00776"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202001172"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c03245"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202408189"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.11.002"}],"container-title":["Small 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