{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T11:18:10Z","timestamp":1783682290125,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32422046"],"award-info":[{"award-number":["32422046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52303167"],"award-info":[{"award-number":["52303167"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52203152"],"award-info":[{"award-number":["52203152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JSGGKQTD20221101115654021"],"award-info":[{"award-number":["JSGGKQTD20221101115654021"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["RCBS20221008093222008"],"award-info":[{"award-number":["RCBS20221008093222008"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small Methods"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The mechanical properties of oocytes are regarded as important indicators of their developmental potential. During fertilization, deviations from the normal mechanical range can hinder sperm penetration, ultimately reducing fertilization efficiency and compromising embryo quality. However, current methods for measuring oocyte mechanics often suffer from low automation levels and predominantly provide local, rather than whole\u2010cell, mechanical characterization. To address these limitations, we developed an AI\u2010guided \u00b5N\u2010scale mechanical measurement instrument for safe and automated oocyte quality assessment. The instrument integrates voice interaction with automated experimental workflows to control a magnetically actuated microgripper, which applies defined loading forces to induce micron\u2010scale compressive deformation of the oocyte. Combined with AI\u2010assisted object detection and image segmentation algorithms, the instrument captures cellular deformation in real time, enabling precise calculation of the oocyte's compressive modulus. This measurement instrument enables automated, quantitative, and non\u2010destructive evaluation of oocyte mechanical properties, providing an effective approach for oocyte quality screening in in vitro fertilization (IVF) and other assisted reproductive technologies (ART).<\/jats:p>","DOI":"10.1002\/smtd.202502390","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T10:44:19Z","timestamp":1778064259000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An AI\u2010Guided Mechanotyping Instrument for Fully Automated Oocyte Quality Assessment"],"prefix":"10.1002","volume":"10","author":[{"given":"Yining","family":"Guo","sequence":"first","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshuo","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"},{"name":"College of Mechanical Engineering Hubei University of Technology  Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueying","family":"Sun","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"},{"name":"College of Electronic and Optical Engineering &amp; College of Flexible Electronics (Future Technology) Nanjing University of Posts and Telecommunications  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wujing","family":"Cao","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7999-0814","authenticated-orcid":false,"given":"Haifeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010019\u201001677\u20106"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2019.06.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.12.115"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adr9869"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmx040"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10809"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3517"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.01.051"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2799"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015470\u2010y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaw071"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201021\u20100122"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2012.163"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202500221"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c6ib00044d"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2012.0269"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42254\u2010018\u20100001\u20107"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10294"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaw019"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10\u201001\u20100066"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109290(99)00175\u2010X"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010021\u201002121\u20101"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3772"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1424"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1342905"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201017\u20100230"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top078600"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2013.05.013"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c11507"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201004220\u20108"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.113076"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.05.072"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2025.02.004"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.02.018"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10404\u2010015\u20101614\u20100"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00595a"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj1133"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi6111457"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010018\u20100015\u20101"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.59717\/j.xinn\u2010med.2024.100055"},{"key":"e_1_2_9_43_1","first-page":"1","author":"Jocher G.","year":"2020","journal-title":"ETD UGM"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743\u20101050\u20103\u20102"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.095281"},{"key":"e_1_2_9_46_1","doi-asserted-by":"crossref","unstructured":"H.Hertz 1882(1882):156.","DOI":"10.1515\/crll.1882.92.156"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1115\/1.2903407"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10237\u2010020\u201001397\u20102"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7027"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.12717\/dr.2018.22.3.245"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1713395115"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010023\u201001567\u20100"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113521"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0lc00840k"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201067638\u2010z"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c09753"}],"container-title":["Small Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.202502390","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smtd.202502390","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smtd.202502390","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T11:08:24Z","timestamp":1783681704000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smtd.202502390"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,6]]},"references-count":55,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/smtd.202502390"],"URL":"https:\/\/doi.org\/10.1002\/smtd.202502390","archive":["Portico"],"relation":{},"ISSN":["2366-9608","2366-9608"],"issn-type":[{"value":"2366-9608","type":"print"},{"value":"2366-9608","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,6]]},"assertion":[{"value":"2025-11-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02390"}}